The amelioration of streptozotocin diabetes-induced renal damage by Wu-Ling-San (Hoelen Five Herb Formula), a traditional Chinese prescription
The amelioration of streptozotocin diabetes-induced renal damage by Wu-Ling-San (Hoelen Five Herb Formula), a traditional Chinese prescription
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DOI：
10.1016/j.jep.2009.04.021
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发表时间：
2009-07-15
影响因子：
5.4
通讯作者：
Chang, Chia Ju
中科院分区：
文献类型：
--
作者：
Liu, I-Min;Tzeng, Thing-Fong;Chang, Chia Ju
民族药理学意义：五灵散是一种促进水代谢的中药方剂。目的：研究五灵散对糖尿病肾病的影响。材料和方法：给链脲佐菌素(STZ)糖尿病大鼠灌胃五灵散(0.5，1.5，2.5g/(kg·d))，每日1次，连续10周。观察给药10周后大鼠血、尿肾功能相关指标的变化。结果：乌灵散2.5g/(kg·d)能部分降低糖尿病大鼠的高血糖水平。五灵散可改善糖尿病相关性尿白蛋白、24小时尿白蛋白排泄率、内生肌酐清除率和肾小球系膜基质扩张。五灵散可减轻糖尿病大鼠肾脏核因子-kB、转化生长因子-β1的表达增加和纤维连接蛋白的进行性积聚。五灵散不仅能改善糖尿病大鼠肾脏晚期糖基化终产物(AGEs)和线粒体硫代巴比妥酸活性物质的升高，而且还能改善糖尿病大鼠肾脏N-epsilon-(羧甲基)赖氨酸和AGEs受体的高水平。结论：五灵散具有抗糖尿病作用，其降糖作用与减轻糖基化所致的肾损伤有关。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Ethnopharmacological relevance: Wu-Ling-San (Hoelen Five Herb Formula) is a Chinese prescription used to promote water metabolism.Aim of the study: The present study was undertaken to characterize the effects of Wu-Ling-San on diabetic nephropathy.Materials and methods: Streptozotocin (STZ)-diabetic rats were orally administrated with Wu-Ling-San (0.5, 1.5, 2.5 g/(kg day)) once a day for 10 weeks. Changes in the renal function related parameters in plasma and urine were analyzed at the end of 10-week administration. Kidney was isolated for pathology histology, immunohistochemistry staining and Western blot analyses.Results: Wu-Ling-San (2.5 g/(kg day)) partially decreased the high plasma glucose level in diabetic rats. Diabetic-dependent alterations in urinary albumin, 24-h urinary albumin excretion rate, creatinine clearance, and glomerular mesangial matrix expansion were ameliorated by Wu-Ling-San. The increased expression of nuclear factor-kB as well as transforming growth factor-P I and the progressive accumulation of fibronectin in kidney of diabetic rats were attenuated by Wu-Ling-San. Not only the elevated levels of advanced glycation end products (AGEs) and mitochondrial thiobarbituric acid-reactive substance, but also the higher levels of N-epsilon-(carboxymethyl)lysine and receptor for AGEs in kidney of diabetic rats were ameliorated by Wu-Ling-San.Conclusions: : Wu-Ling-San posses an anti-diabetic property with plasma glucose lowering action accompanied with amelioration of glycation-mediated renal damage. (C) 2009 Elsevier Ireland Ltd. All rights reserved.