REGULATION OF HUMAN INTERLEUKIN-2 GENE - FUNCTIONAL DNA-SEQUENCES IN THE 5' FLANKING REGION FOR THE GENE-EXPRESSION IN ACTIVATED LYMPHOCYTES-T
REGULATION OF HUMAN INTERLEUKIN-2 GENE - FUNCTIONAL DNA-SEQUENCES IN THE 5' FLANKING REGION FOR THE GENE-EXPRESSION IN ACTIVATED LYMPHOCYTES-T
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DOI：
10.1016/0092-8674(86)90660-4
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发表时间：
1986-08-01
期刊：
影响因子：
64.5
通讯作者：
TANIGUCHI, T
中科院分区：
文献类型：
--
作者：
FUJITA, T;SHIBUYA, H;TANIGUCHI, T
白细胞介素-2（IL-2）是一种淋巴因子，在免疫系统中发挥着至关重要的作用，尤其是在T淋巴细胞的生长控制中。这种淋巴因子的表达仅限于活化的T淋巴细胞。在这里，我们证明了在人IL-2基因的5“侧翼区域中存在独特的DNA序列，其控制诱导的T细胞特异性基因表达。我们还表明，DNA序列的功能在一个方向独立的方式和激活异源启动子，否则在诱导的T细胞是惰性的。该DNA跨越约200 bp，含有与HTLV-III（或LAV）的LTR序列和IL-2受体和干扰素-γ的5“上游区具有序列同源性的区域。基因.
Interleukin-2 (IL-2) is a lymphokine that plays a crucial role in the immune system, especially in the growth control of T lymphocytes. Expression of this lymphokine is restricted to activated T lymphocytes. Here we demonstrate the presence of unique DNA sequences in the 5'' flanking region of the human IL-2 gene that control induced T-cell-specific gene expression. We also show that the DNA sequences function in an orientation-independent manner and activate a heterologous promoter which is otherwise inert in induced T cells. The DNA, which spans about 200 bp, contains regions with sequence homology to LTR sequences of HTLV-III (or LAV) and the 5'' upstream region of the IL-2 receptor and interferon-.gamma. genes.