Formation of actin stress fibers and focal adhesions enhanced by Rho-kinase
Formation of actin stress fibers and focal adhesions enhanced by Rho-kinase
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DOI：
10.1126/science.275.5304.1308
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发表时间：
1997-02-28
期刊：
影响因子：
56.9
通讯作者：
Kaibuchi, K
中科院分区：
文献类型：
--
作者：
Amano, M;Chihara, K;Kaibuchi, K
小的鸟苷三磷酸酶（GTdR）Rho与由细胞外信号如溶血磷脂酸（LPA）刺激的成纤维细胞中的应力纤维和粘着斑的形成有关。Rho激酶可被Rho激活，并可能介导Rho的某些生物学效应。微量注射Rho激酶的催化结构域到血清饥饿的瑞士3T3细胞诱导应力纤维和局灶性粘连的形成，而微量注射非活性催化结构域，Rho结合结构域，或pleckstrin同源结构域抑制LPA诱导的应力纤维和局灶性粘连的形成。因此，Rho激酶似乎介导来自Rho的信号并诱导应力纤维和局灶性粘连的形成。
The small guanosine triphosphatase (GTPase) Rho is implicated in the formation of stress fibers and focal adhesions in fibroblasts stimulated by extracellular signals such as lysophosphatidic acid (LPA). Rho-kinase is activated by Rho and may mediate some biological effects of Rho. Microinjection of the catalytic domain of Rho-kinase into serum-starved Swiss 3T3 cells induced the formation of stress fibers and focal adhesions, whereas microinjection of the inactive catalytic domain, the Rho-binding domain, or the pleckstrin-homology domain inhibited the LPA-induced formation of stress fibers and focal adhesions. Thus, Rho-kinase appears to mediate signals from Rho and to induce the formation of stress fibers and focal adhesions.