Retraction ATPase Motors from Three Orthologous Type IVa Pilus Systems Support Promiscuous Retraction of the Vibrio cholerae Competence Pilus.
Retraction ATPase Motors from Three Orthologous Type IVa Pilus Systems Support Promiscuous Retraction of the Vibrio cholerae Competence Pilus.
复制标题
来自三个直系同源 IVa 型菌毛系统的回缩 ATP 酶马达支持霍乱弧菌能力菌毛的混杂回缩。
DOI：
10.1128/jb.00126-22
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发表时间：
2022
影响因子：
3.2
通讯作者：
Dalia,AnkurB
中科院分区：
文献类型：
--
作者：
Couser,Evan;Chlebek,JenniferL;Dalia,AnkurB
被称为IVa型皮利（T4aP）的细菌表面附属物促进多种活动，包括DNA摄取、抽搐运动和毒力。这些活动依赖于T4aP从细胞表面动态延伸和缩回的能力。动态延伸依赖于通常称为PilB的马达ATP酶。大多数T4 aP还依赖于特定的运动ATP酶，通常称为PilT和皮鲁，以动态和有力地缩回。在这里，我们系统地评估是否电机ATP酶从三个orthopathic T4aP功能互补霍乱弧菌突变体，缺乏其内源性马达。我们发现，来自所测试的三个T4 aP系统的PilT和皮鲁收缩ATP酶是混杂的，并且促进霍乱弧菌感受态T4 aP的收缩，尽管存在高度的序列差异。相比之下，来自相同T4aP系统的邻位延伸ATP酶不能介导霍乱弧菌感受态T4aP的延伸，尽管表现出相似程度的序列差异。此外，我们表明，特征之一的PilT的直系同源物不支持PilU依赖的回缩，并提供了一些数据，表明PilT的C末端是重要的PilU依赖的回缩。总之，我们的数据表明，收缩ATP酶可能保持了高度的滥交，促进收缩的T4aP，而延伸ATP酶可能已经演变成为特定的同源system.IMPORTANCEOne的方式，其中细菌与他们的环境相互作用是通过头发样的附属物称为IVa型皮利（T4aP）。这些附属物通过不同的ATP酶马达的作用从细胞表面动态地延伸和缩回。T4aP存在于多种细菌物种中。在这里，我们表明，从三个T4aP的缩回马达是混杂的，能够促进异源T4aP系统的缩回。相反，来自这些相同T4aP系统的延伸ATP酶马达是特异性的，并且不能促进异源T4aP的延伸。因此，这些结果表明，T4aP延伸可能比T4aP收缩更严格地调节。
Bacterial surface appendages called type IVa pili (T4aP) promote diverse activities, including DNA uptake, twitching motility, and virulence. These activities rely on the ability of T4aP to dynamically extend and retract from the cell surface. Dynamic extension relies on a motor ATPase commonly called PilB. Most T4aP also rely on specific motor ATPases, commonly called PilT and PilU, to dynamically and forcefully retract. Here, we systematically assess whether motor ATPases from three orthologous T4aP can functionally complement Vibrio cholerae mutants that lack their endogenous motors. We found that the PilT and PilU retraction ATPases from the three T4aP systems tested are promiscuous and promote the retraction of the V. cholerae competence T4aP despite a high degree of sequence divergence. In contrast, the orthologous extension ATPases from the same T4aP systems were not able to mediate the extension of the V. cholerae competence T4aP despite exhibiting a similar degree of sequence divergence. Also, we show that one of the PilT orthologs characterized does not support PilU-dependent retraction and provide some data to indicate that the C terminus of PilT is important for PilU-dependent retraction. Together, our data suggest that retraction ATPases may have maintained a high degree of promiscuity for promoting the retraction of T4aP, while extension ATPases may have evolved to become specific for their cognate systems.IMPORTANCEOne way in which bacteria interact with their environments is via hair-like appendages called type IVa pili (T4aP). These appendages dynamically extend and retract from the cell surface via the action of distinct ATPase motors. T4aP are present in diverse bacterial species. Here, we demonstrate that retraction motors from three T4aP are promiscuous and capable of promoting the retraction of a heterologous T4aP system. In contrast, the extension ATPase motors from these same T4aP systems are specific and cannot promote the extension of a heterologous T4aP. Thus, these results suggest that T4aP extension may be more tightly regulated than T4aP retraction.