Indirect Method for Quantification of Cell Biomass During Solid-State Fermentation of Palm Kernel Cake Based on Protein Content
Indirect Method for Quantification of Cell Biomass During Solid-State Fermentation of Palm Kernel Cake Based on Protein Content
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基于蛋白质含量的棕榈仁饼固态发酵过程中细胞生物量的间接定量方法
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
N. Samat
中科院分区：
文献类型：
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作者：
S. Abd;G. Hung;M. Hassan;M. Karim;N. Samat
尼日尔FTCC 5003固态发酵棕榈仁饼是棕榈仁饼高附加值化的有效途径。本研究是在尼日尔FTCC 5003的生长曲线和模型，能够描述在SSF使用PKC底物的生长进行。针对SSF体系中细胞生物量难以从底物中定量分离的问题，以葡萄糖胺和蛋白质含量为基础，研究了间接测定尼日尔FTCC 5003 SSF过程中细胞生长的方法。葡萄糖胺和蛋白质含量与真菌细胞干重（DW）之间的初步关系，使用模拟均匀的SSF数据，使用玻璃珠作为支持材料。葡萄糖胺和蛋白质含量都与蛋白质和葡萄糖胺支持材料上的SSF中的真菌细胞干重有很好的相关性。将得到的方程用于从葡萄糖胺和蛋白质作为生长指示剂研究的数据估计PKC SSF过程中的细胞生物量分布。基于蛋白质浓度和I²-甘露聚糖酶活性估算的真菌干细胞重与PKC干细胞重具有良好的相关性，表明两者都是描述A.尼日尔FTCC 5003在该系统中。与此相反，基于葡萄糖胺浓度的真菌干细胞重量估计不适合描述A.尼日尔FTCC 5003。
Solid-State Fermentation (SSF) of Aspergillus niger FTCC 5003 on Palm Kernel Cake (PKC) is a practical approach to upgrade PKC into value added product. Present study was conducted on Aspergillus niger FTCC 5003 growth profile and models that are able to describe the growth in SSF using PKC substrate. Due to the difficulties of separating cell biomass quantitatively from the substrate for SSF systems, indirect method for measurement of cell growth during SSF of PKC by Aspergillus niger FTCC 5003 was studied based on the estimation of glucosamine and protein content. Preliminary relationships between glucosamine and protein contents to fungal dry cell weight (Dw) were developed using simulated homogenous SSF data using glass beads as support materials. Both glucosamine and protein contents were well correlated to the fungal dry cell weight in SSF on support materials for protein and glucosamine, respectively. The equations obtained were used for the estimation of cell biomass profile during SSF of PKC from the data of glucosamine and protein as growth indicator study. The estimated fungal dry cell weight based on protein concentration and I²-mannanase activity as metabolic activity for microbial growth were well correlated to PKC dry weight which, indicating that both were suitable marker in describing the growth of A. niger FTCC 5003 in this system. In contrast, estimated fungal dry cell weight based on glucosamine concentration was not suitable to describe the growth of A. niger FTCC 5003.