Circulating Cell Free Mitochondrial DNA is a Biomarker in the Development of Coronary Heart Disease in the Patients with Type 2 Diabetes
Circulating Cell Free Mitochondrial DNA is a Biomarker in the Development of Coronary Heart Disease in the Patients with Type 2 Diabetes
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循环无细胞线粒体 DNA 是 2 型糖尿病患者发生冠心病的生物标志物
DOI：
10.7754/clin.lab.2014.141132
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发表时间：
2015-01-01
影响因子：
0.7
通讯作者：
Gong, Jianbin
中科院分区：
文献类型：
--
作者：
Liu, Jing;Cai, Xiaomin;Gong, Jianbin
背景：循环中的无细胞线粒体DNA(CCF-mtDNA)可能来源于体内受损的组织或细胞，已被认为是多种疾病的潜在生物标志物。本研究旨在探讨CCF-mtDNA水平能否作为糖尿病无冠心病(CHD)患者的生物标志物，并与其发病相关。方法：检测50例2型糖尿病(DM)合并CHD患者、50例DM非CHD患者和50例年龄、性别匹配的健康对照组血浆CCF-mtDNA水平及传统冠心病危险因素。线粒体DNA水平通过实时定量聚合酶链式反应检测NADH脱氢酶1基因来评估。同时测定糖尿病伴或不伴冠心病患者血浆线粒体DNA的ROC曲线。结果：糖尿病组血浆CCF-mtDNA水平和C反应蛋白水平均显著高于健康对照组，糖尿病合并冠心病组mtDNA水平与非冠心病组比较差异有统计学意义。糖尿病合并冠心病组与正常对照组相比，线粒体DNA的ROC曲线下面积分别为0.843和0.898。相关分析显示冠心病患者mtDNA水平与C-反应蛋白(CRP)水平显著相关。结论：2型糖尿病合并CHD患者CCF-mtDNA水平升高，且与CRP水平呈正相关。两者都可以被认为是CHID发展的一个预后生物标志物。
Background: Circulating cell-free mtDNA (ccf-mtDNA) is presumably derived from injured tissue or cells in the body and has been suggested to be a potential biomarker in several diseases. In the present study, we investigated whether ccf-mtDNA levels could serve as a biomarker of diabetic patients without Coronary Heart Disease (CHD) and correlate with their incidence to develop CHD.Methods: Ccf-mtDNA levels in plasma and the traditional CHD risk factors were determined in 50 type 2 diabetes (DM) with CHD and 50 DM without CHD patients and 50 age- and sex-matched healthy controls. MtDNA levels were assessed by measuring the NADH dehydrogenase 1 gene using quantitative real-time PCR. ROC curve analyses of plasma mtDNA in DM with or without CHD were also determined. Multivariate logistic regression analyses were conducted to determine the correlation between the mtDNA levels and traditional CHD risk factors.Results: The plasma ccf-mtDNA levels and CRP were significantly elevated in DM compared with healthy controls, and a consistent increase of mtDNA in diabetes with CHD compared to those without CHD. The area under the ROC curves of mtDNA in DM patients versus DM patients with CHD and healthy controls was 0.843 and 0.898, respectively. Correlation analyses showed that the mtDNA levels were significantly correlated with that of C-reactive protein (CRP) in patients with CHD.Conclusions: Ccf-mtDNA was elevated in type 2 diabetes with CHD and correlated with CRP levels. Both may be considered a prognostic biomarker of CHID development.