MAP kinase activation by mu opioid receptor involves phosphatidylinositol 3-kinase but not the cAMP/PKA pathway
MAP kinase activation by mu opioid receptor involves phosphatidylinositol 3-kinase but not the cAMP/PKA pathway
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DOI：
10.1016/s0014-5793(99)00949-7
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发表时间：
1999-07-30
期刊：
影响因子：
3.5
通讯作者：
Yu, L
中科院分区：
文献类型：
--
作者：
Ai, WD;Gong, JH;Yu, L
使用受体克隆转染的细胞研究了丝裂原激活蛋白（MAP）激酶的μ阿片受体激活中蛋白激酶的参与。 cAMP/蛋白激酶A（PKA）途径已知是mu阿片受体信号转导的主要生化途径，然而，我们的数据显示刺激腺苷酸环化酶或激活PKA对mu受体MAP激酶活性增强没有影响，这表明cAMP/PKA。通路不参与介导 MAP、激酶的 mu 受体激活，抑制磷脂酰肌醇 (PI) 3-激酶可降低 mu 受体，增强 MAP 激酶活性，表明 IPI 3-激酶参与其中，总而言之，这些结果表明 mu 阿片受体和 MAP 激酶级联之间的串扰不是由 cAMP/PKA 通路介导的，而是涉及 PI 3-激酶，(C) 1999 年欧洲生化学会联合会。
The involvement of protein kinases was studied in mu opioid receptor activation of mitogen-activated protein (MAP) kinase using cells transfected with the receptor clone. The cAMP/protein kinase A (PKA) pathway is known to be the major biochemical pathway For mu opioid receptor signaling, However, our data showed that stimulating adenylyl cyclase or activating PKA had no effect on mu receptor enhancement of MAP kinase activity, suggesting that the cAMP/PKA. pathway is not involved in mediating the mu receptor activation of MAP, kinase, Inhibition of phosphatidylinositol (PI) 3-kinase reduced mu receptor enhancement of MAP kinase activity, suggesting IPI 3-kinase involvement, Together, these results show that crosstalk between the mu opioid receptor and the MAP kinase cascade is not mediated by the cAMP/PKA pathway, but involves PI 3-kinase, (C) 1999 Federation of European Biochemical Societies.