OVULATION IN RATS FROM PREOPTIC STIMULATION THROUGH PLATINUM ELECTRODES . IMPORTANCE OF DURATION AND SPREAD OF STIMULUS
OVULATION IN RATS FROM PREOPTIC STIMULATION THROUGH PLATINUM ELECTRODES . IMPORTANCE OF DURATION AND SPREAD OF STIMULUS
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DOI：
10.1210/endo-76-6-1195
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发表时间：
1965-01-01
期刊：
影响因子：
4.8
通讯作者：
EVERETT, JW
中科院分区：
文献类型：
--
作者：
EVERETT, JW
处于发情前期的成年雌性大鼠（其自发排卵被戊巴比妥阻断）通过铂电极刺激，该电极横跨大脑内侧视前区，两侧间隔2 mm。1毫秒矩形脉冲的双相对，1毫安峰-峰，30/秒，以30秒的间隔以30秒的序列通过。在所有8只大鼠中，刺激60 min过夜均发生完全排卵（9-12个卵子）。较短的刺激（15-30 min）导致排卵和卵子数量较少的大鼠。排卵失败（5只大鼠）时，刺激仅持续10分钟。一个输卵管卵子5只大鼠中获得后，电极简单地插入60分钟。刺激60分钟。通过外侧视前区给大多数阴性结果。除了证明“电”刺激而不是之前采用的铁电解沉积的可行性外，结果还强调了时间的重要性以及穿过内侧视前脑的假设弥漫性神经元系统在控制[促亮化激素]量方面的参与程度腺垂体释放的“LH”。某些影响提到其他物种的尝试，以诱导排卵的脑刺激。
Adult female rats in proestrus, their spontaneous ovulation blocked by pentobarbital, were stimulated through platinum electrodes placed bilaterally 2 mm apart across the medial preoptic areas of the brain. Biphasic pairs of 1 msec rectangular pulses, 1 mamp peak-to-peak, 30/sec, were passed in 30-sec trains at 30-sec intervals. Full ovulation (9-12 ova) occurred overnight in all of 8 rats thus stimulated for 60 min. Shorter stimulation (15-30 min) led to fewer rats ovulating and smaller numbers of ova. Ovulation failed (5 rats) when stimulation lasted only 10 min. One tubal ovum among 5 rats was obtained after electrodes were simply inserted for 60 min. Stimulation for 60 min. across the lateral preoptic area gave mostly negative results. Besides demonstrating the feasibility of stimulating "electrically" rather than by the electrolytic deposition of iron previously employed, the results emphasize the importance of time and of the degree of involvement of a postulated diffuse neuronal system traversing the medial preoptic brain in governing the amount of [leutinizing hormone] "LH" released by the adeno-hypophysis. Certain implications are mentioned concerning attempts in other species to induce ovulation by brain stimulation.