REGULATION OF AMELOGENIN GENE-EXPRESSION DURING TOOTH DEVELOPMENT
REGULATION OF AMELOGENIN GENE-EXPRESSION DURING TOOTH DEVELOPMENT
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DOI：
10.1002/aja.1001990304
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发表时间：
1994-03-01
影响因子：
2.5
通讯作者：
GIBSON, CW
中科院分区：
文献类型：
--
作者：
CHEN, EH;PIDDINGTON, R;GIBSON, CW
淀粉原蛋白是牙釉质发育过程中主要的基质蛋白，对牙釉质矿化至关重要。构建转基因小鼠，以鉴定淀粉原蛋白基因在牙釉质器官细胞中特异性表达所需的片段。一个3.5 kb的牛x染色体淀粉原基因片段，包括一个TATA盒子、转录起始位点和32 bp的外显子1，与β -半乳糖苷酶基因连接，并注射到受精卵中。新生转基因阳性小鼠在用显色底物Xgal处理的发育牙齿中表达了β -半乳糖苷酶活性。新生小鼠成釉细胞灶阳性；小鼠出生后第1天和第2天染色强度和阳性成釉细胞数量增加。部分相邻层的中间细胞在后期也呈阳性。转基因对牙釉质器官的靶向性是特异性的；唯一观察到阳性的其他细胞是巨噬细胞，它们具有内源性β -半乳糖苷酶活性。(C) 1994 Wiley-Liss, Inc。
The amelogenins are the predominant matrix proteins in developing enamel and are crucial for proper enamel mineralization. Transgenic mice were constructed in order to identify the segment of the amelogenin gene required for specific expression in enamel organ cells. A 3.5 kb fragment of the bovine X-chromosomal amelogenin gene that includes a TATA box, the transcription initiation site, and 32 bp of exon 1 was linked to the betagalactosidase gene and injected into fertilized mouse eggs. Newborn transgene positive mice expressed betagalactosidase activity in developing teeth treated with the chromogenic substrate Xgal. Foci of ameloblasts were positive in newborn mice; stain intensity and number of positive ameloblasts increased in 1-day and 2-day postnatal mice. Some of the adjacent stratum intermedium cells also were positive in the tater stages. Targeting of the transgene to the enamel organ was specific; the only other cells observed to be positive were macrophages, which have endogenous betagalactosidase activity. (C) 1994 Wiley-Liss, Inc.