Immunohistochemical localisation of vitamin B12 R-binder in the human digestive tract.
Immunohistochemical localisation of vitamin B12 R-binder in the human digestive tract.
复制标题
维生素 B12 R 结合剂在人体消化道中的免疫组织化学定位。
作者：
H. Kudo;M. Inada;G. Ohshio;Y. Wakatsuki;K. Ogawa;Y. Hamashima;T. Miyake
采用间接免疫过氧化物酶技术研究了维生素B12 r -结合剂在人消化道中的分布。唾液腺和食管腺的黏液细胞和导管上皮细胞R-binder染色阳性。正常胃黏膜R-binder未见阳性染色，而肠化生区柱状上皮细胞和杯状细胞染色阳性。胆囊、肝内胆管和胰管上皮细胞R-binder阳性。在小肠和结肠的柱状上皮细胞和杯状细胞中发现R-binder。肠黏膜提取物中不饱和维生素B12结合能力和钴胺素含量的测定也表明肠黏膜中存在r - binding。
The distribution of vitamin B12 R-binder in the human digestive tract was studied using an indirect immunoperoxidase technique. Positive staining for R-binder was found in the mucous cells and ductal epithelial cells of the salivary glands and the oesophageal glands. In normal gastric mucosa, no positive staining for R-binder was found, but in the area with intestinal metaplasia, the columnar epithelial cells and goblet cells showed positive staining. Epithelial cells of the gallbladder, intrahepatic bile ducts and pancreatic ducts were also positive for R-binder. In the small intestine and colon, R-binder was found in the columnar epithelial cells and goblet cells. The measurement of unsaturated vitamin B12 binding capacity and cobalamin content in the extracts from intestinal mucosa also indicated the presence of R-binder in the intestinal mucosa.