Parathyroid hormone (PTH) secretion, PTH mRNA and calcium-sensing receptor mRNA expression in equine parathyrold cells, and effects of interleukin (IL)-1 IL-6, and tumor necrosis factor-α on equine parathyroid cell function
Parathyroid hormone (PTH) secretion, PTH mRNA and calcium-sensing receptor mRNA expression in equine parathyrold cells, and effects of interleukin (IL)-1 IL-6, and tumor necrosis factor-α on equine parathyroid cell function
复制标题
DOI：
10.1677/jme.0.0310609
复制
发表时间：
2003-12-01
影响因子：
3.5
通讯作者：
Rosol, TJ
中科院分区：
文献类型：
--
作者：
Toribio, RE;Kohn, CW;Rosol, TJ
甲状旁腺激素（PTH）是由甲状旁腺的主细胞响应于离子钙（Ca 2+）浓度的变化而分泌的。在这项研究中，我们测定了PTH分泌，PTH mRNA和钙敏感受体（CaR）mRNA的表达由马甲状旁腺主细胞在体外。我们还评估了白细胞介素（IL）-1 β、IL-6和肿瘤坏死因子（TNF）-α对PTH分泌以及PTH和CaR mRNA表达的影响。PTH与Ca ~（2+）呈负相关。PTH分泌从100%（第0天）降至13%（第30天）。PTH mRNA表达从100%（第0天）下降到25%（第30天）。CaR mRNA从100%（第0天）下降到16%（第30天）。主细胞暴露于高（2（.）0 mM）的Ca 2+浓度与低Ca 2+浓度相比，PTH mRNA表达降低。Ca 2+浓度对CaR mRNA表达无影响。高Ca 2+浓度对PTH分泌的抑制作用也随着时间的推移而下降。第10天后，低和高钙浓度之间的PTH分泌没有显着差异。IL-1 β使PTH分泌和PTH mRNA表达均降低（75%和73%），并导致CaR mRNA显著过表达（高达142%）。IL-1 β的作用可被IL-1受体拮抗剂阻断。IL-1 P使Ca 2+设定点从1.4 mM降低至1.2 mM。IL-6降低PTH分泌（74%），但对PTH和CaR mRNA表达无影响。TNF-α对PTH分泌、PTH和CaR mRNA表达无影响。总之，甲状旁腺细胞从0到30天对Ca 2+的反应性降低可以部分解释为CaR表达降低。IL-1 β和IL-6，而不是TNF-α影响甲状旁腺功能在体外，可能是重要的影响PTH分泌在败血症的马。
Parathyroid hormone (PTH) is secreted by the chief cells of the parathyroid gland in response to changes in ionized calcium (Ca2+) concentrations. In this study, we measured PTH secretion, and PTH mRNA and calcium-sensing receptor (CaR) mRNA expression by equine parathyroid chief cells in vitro. We also evaluated the effects of interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha on PTH secretion, and PTH and CaR mRNA expression. The relationship between PTH and Ca2+ was inversely related. PTH secretion decreased from 100% (day 0) to 13% (day 30). PTH mRNA expression declined from 100% (day 0) to 25% (day 30). CaR mRNA decreased from 100% (day 0) to 16% (day 30). Chief cells exposed to high (2(.)0 mM) Ca2+ concentrations had a lower PTH mRNA expression compared with low Ca2+ concentrations. Ca2+, concentrations had no effect on CaR mRNA expression. The inhibitory effect of high Ca2+ concentrations on PTH secretion also declined over time. After day 10, there was no significant difference in PTH secretion between low and high Ca2+ concentrations. IL-1beta decreased both PTH secretion (75%) and PTH mRNA expression (73%), and resulted in a significant overexpression of CaR mRNA (up to 142%). The effects of IL-1beta were blocked by an IL-1 receptor antagonist. IL-1 P decreased the Ca2+ set-point from 1.4 mM to 1.2 mM. IL-6 decreased PTH secretion (74%), but had no effect on PTH and CaR mRNA expression. TNF-alpha had no effect on PTH secretion, and PTH and CaR mRNA expression. In summary, the decreased responsiveness of parathyroid cells to Ca2+ from 0 to 30 days can be explained, in part, by the reduced CaR expression. IL-1beta and IL-6 but not TNF-a affected parathyroid function in vitro and may be important in influencing PTH secretion in the septic horse.