Expression of Podoplanin and Other Lymphatic Markers in the Human Anterior Eye Segment
Expression of Podoplanin and Other Lymphatic Markers in the Human Anterior Eye Segment
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DOI：
10.1167/iovs.08-3307
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发表时间：
2010-01-01
影响因子：
4.4
通讯作者：
Birke, Marco T.
中科院分区：
文献类型：
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作者：
Birke, Kerstin;Luetjen-Drecoll, Elke;Birke, Marco T.
目的。目的探讨淋巴管内皮细胞(LEC)标记物在眼前段组织中的表达。方法：对8例(37~100岁)供者的8只眼的眼前段组织切片进行血管内皮生长因子-R3、Prox-1、LYVE-1、Pdpn、PCX、CCR7、Ccl19、CCL21和CD68的染色。用免疫金标记法对5只眼进行PDPN定位分析。用RT-PCR方法检测3只眼的虹膜和小梁网(TM)组织中LEC标志物和趋化因子受体/配体的表达。结果VEGF-R3和Prox-1在眼前节无结构表达。LYVE-1染色的单个树突状细胞位于睫状体、TM和虹膜。Pdpn对所有小梁细胞、纤毛小梁细胞和睫状肌最前面的肌周细胞进行染色。Schlemm管内皮细胞未被染色，但可与Podocalyin抗体反应。虹膜间质仅见单个树突状细胞，前表面几乎所有细胞均为Pdpn(+)。少数基质细胞为Pdpn(+)/Lyve(+)，少数前表面细胞为Pdpn(+)/CCL21(+)。也可见孤立的CCR7(+)细胞。如果结果经PCR证实，但在TM和虹膜中检测到Prox-1。结论Pdpn和CCL21在虹膜前表面和房角的共同表达提示存在趋化因子梯度，引导APC穿过前房。Pdpn在TM中的表达越明显，可能有利于APC通过常规流出的方式流出。(投资眼科VS科学。2010年；51：344-354)doi：10.1167/iovs.08-3307
PURPOSE. To investigate the expression of lymphatic endothelial cell (LEC) markers in tissues of the anterior eye segment.METHODS. Sections of human anterior segments from eight eyes of eight donors (37-100 years) were stained for Vegf-R3, Prox-1, Lyve-1, Pdpn, Pcx, CCR7, Ccl19, Ccl21, and CD68. Pdpn localization was additionally analyzed by immunogold labeling on sections of five eyes. Expression of LEC markers and chemokine receptor/ligands was analyzed by RT-PCR in iris and trabecular meshwork (TM) tissue from three eyes and eight human TM (hTM) cell cultures.RESULTS. Vegf-R3 and Prox-1 stained no structures in the anterior segment. Lyve-1 stained single dendriform cells in the ciliary body, the TM, and the iris. Pdpn stained all trabecular cells, the cells of the trabeculum ciliare, and the anteriormost perimysium cells of the ciliary muscle. Schlemm's canal endothelium was not stained but reacted to a podocalyxin antibody. In the iris stroma, single dendriform cells were stained; at the anterior surface, almost all cells were Pdpn(+). Few stromal cells were Pdpn(+)/Lyve(+), but several anterior surface cells were Pdpn(+)/Ccl21(+). Solitary CCR7(+) cells were observed there, too. IF results were confirmed by PCR, but Prox-1 was detected in TM and iris. Cultured hTM cells displayed partial Pdpn/Ccl21 colocalization.CONCLUSIONS. Coexpression of Pdpn and Ccl21 at the anterior iris surface and in the chamber angle suggests the constitution of a chemokine gradient guiding APCs through the anterior chamber. The more pronounced expression of Pdpn in the TM could favor egression of APCs by way of the conventional outflow. (Invest Ophthalmol Vis Sci. 2010; 51:344-354) DOI:10.1167/iovs.08-3307