Changes in the transcriptome of bovine ovarian cortex during follicle activation in vitro.
Changes in the transcriptome of bovine ovarian cortex during follicle activation in vitro.
复制标题
体外卵泡激活过程中牛卵巢皮质转录组的变化。
DOI：
10.1152/physiolgenomics.00060.2015
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发表时间：
2015
影响因子：
4.6
通讯作者：
Fortune,JE
中科院分区：
文献类型：
--
作者：
Yang,MY;Fortune,JE
调节激活的信号，卵巢卵泡发育的关键过渡，仍然没有得到很好的理解，特别是在非啮齿动物物种。为了深入了解牛这种转变的调控，我们将微阵列方法与体外系统相结合，在体外系统中，在对照培养基中培养的卵巢皮质片富含原始卵泡，而用胰岛素培养的片富含初级卵泡。从培养的皮质块中提取总RNA，然后使用Affyssin Bovine Genome GeneChip阵列鉴定和分析转录物。牛基因芯片中大约65%的转录本在培养的皮质块中被检测到。与胰岛素或无胰岛素培养的碎片之间的比较产生了158个差异表达的转录本。与对照组相比，90个转录上调，68个下调胰岛素。这些转录物参与许多生物过程和功能，但大多数与细胞生长或细胞周期/细胞死亡有关。编码泛素结合酶E2 C（UBE 2C）的转录本在卵泡激活过程中显著上调，并且不稳定性通路分析显示UBE 2C可以与肿瘤抑制因子磷酸酶和张力蛋白同源物（PTEN）相互作用。与对照组相比，胰岛素处理的皮质块中PTENmRNA和蛋白表达均降低。此外，FOXO 3a，一个下游效应的PTEN信号，经历了核质穿梭在原始初级卵泡发育过程中的牛胎儿卵巢，进一步表明在牛卵泡激活的PTEN途径的参与。在这项研究中发现的基因和途径为进一步研究卵泡激活的机制提供了有趣的候选人。
The signals that regulate activation, a key transition in ovarian follicular development, are still not well understood, especially in nonrodent species. To gain insight into the regulation of this transition in cattle, we combined a microarray approach with an in vitro system in which ovarian cortical pieces cultured in control medium are enriched for primordial follicles, whereas pieces cultured with insulin are enriched for primary follicles. Total RNA was extracted from cultured cortical pieces, and then transcripts were identified and analyzed using the Affymetrix Bovine Genome GeneChip array. Around 65% of the transcripts in the bovine GeneChip were detected in cultured cortical pieces. Comparison between pieces cultured with or without insulin generated 158 differentially expressed transcripts. Compared with controls, 90 transcripts were upregulated and 68 were downregulated by insulin. These transcripts are involved in many biological processes and functions, but most are associated with cellular growth or cell cycle/cell death. The transcript encoding ubiquitin-conjugating enzyme E2C (UBE2C) was significantly upregulated during follicle activation, and Ingenuity Pathways Analysis revealed that UBE2C can interact with the tumor suppressor phosphatase and tensin homolog (PTEN). BothPTENmRNA and protein were lower in cortical pieces cultured with insulin than in controls. In addition, FOXO3a, a downstream effector of PTEN signaling, underwent nuclear-cytoplasmic shuttling during primordial to primary follicle development in bovine fetal ovaries, further suggesting the involvement of the PTEN pathway in follicle activation in cattle. Genes and pathways identified in this study provide interesting candidates for further investigation of mechanisms underlying follicle activation.
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影响因子：
4.8
作者：
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通讯作者：
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原始、初级和次级卵泡早期卵泡发育的基因表达谱。
影响因子：
6.7
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