Check your cultures! A list of cross-contaminated or misidentified cell lines
Check your cultures! A list of cross-contaminated or misidentified cell lines
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DOI：
10.1002/ijc.25242
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发表时间：
2010-07-01
影响因子：
6.4
通讯作者：
Freshney, R. Ian
中科院分区：
文献类型：
--
作者：
Capes-Davis, Amanda;Theodosopoulos, George;Freshney, R. Ian
连续细胞系由来源于特定供体和来源组织的培养细胞组成，这些细胞已经获得了无限增殖的能力。这些细胞系是研究健康和疾病，特别是癌症的公认模型。然而，在使用连续细胞系时需要注意，包括污染的可能性，其中在操作者不知情的情况下引入了外源细胞系或微生物。交叉污染，其中污染物是另一种细胞系，在20世纪50年代首次被认识到，但令人不安的是，今天仍然是一个严重的问题。许多细胞系在早期就被交叉污染，因此随后的实验工作只对污染物进行，并以不同的名称进行伪装。研究人员如何知道自己的细胞系是否交叉污染？在此提出了两个切实可行的对策。首先，重要的是检查文献，寻找以前关于交叉污染的工作。有些报告可能很难找到，为了使这些报告更容易获得，我们编制了一份已知交叉污染细胞系的清单。该清单目前包含360个细胞系，来自68个参考文献。大多数污染物出现在同一物种内，HeLa仍然是人类细胞系中最常见的（29%，106/360），但种间污染物占少数但相当少的病例（9%，33/360）。其次，即使之前没有关于该细胞系交叉污染的出版物，也必须通过执行认证测试来检查样品本身。
Continuous cell lines consist of cultured cells derived from a specific donor and tissue of origin that have acquired the ability to proliferate indefinitely. These cell lines are well-recognized models for the study of health and disease, particularly for cancer. However, there are cautions to be aware of when using continuous cell lines, including the possibility of contamination, in which a foreign cell line or microorganism is introduced without the handler's knowledge. Cross-contamination, in which the contaminant is another cell line, was first recognized in the 1950s but, disturbingly, remains a serious issue today. Many cell lines become cross-contaminated early, so that subsequent experimental work has been performed only on the contaminant, masquerading under a different name. What can be done in response how can a researcher know if their own cell lines are cross-contaminated? Two practical responses are suggested here. First, it is important to check the literature, looking for previous work on cross-contamination. Some reports may be difficult to find and to make these more accessible, we have compiled a list of known cross-contaminated cell lines. The list currently contains 360 cell lines, drawn from 68 references. Most contaminants arise within the same species, with HeLa still the most frequently encountered (29%, 106/360) among human cell lines, but interspecies contaminants account for a small but substantial minority of cases (9%, 33/360). Second, even if there are no previous publications on cross-contamination for that cell line, it is essential to check the sample itself by performing authentication testing.