Flt3 ligand plus IL-7 supports the expansion of murine thymic B cell progenitors that can mature intrathymically.
Flt3 ligand plus IL-7 supports the expansion of murine thymic B cell progenitors that can mature intrathymically.
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Flt3 配体加 IL-7 支持可在胸腺内成熟的小鼠胸腺 B 细胞祖细胞的扩增。
作者：
H. Mckenna;P. Morrissey
Flt 3配体（flt 3L）对造血祖细胞、树突状细胞和B淋巴细胞生成具有有效作用。我们研究了flt 3L对胸腺内前体的影响。向鼠胎儿胸腺叶的叶浸没培养物中添加flt 3L + IL-7导致Thy-1（低）、CD 44（高）、HSA（高）细胞的未成熟群体的扩增。该群体包含具有前体活性的细胞，这取决于其重新填充脱氧鸟苷处理的胎儿胸腺叶的能力。在再进入胸腺叶后，flt 3 L + IL-7培养的Thy-1（低）、CD 44（高）、HSA（高）细胞经历扩增并分化成B细胞。胸腺叶重建后胎儿胸腺器官培养2周后，胸腺内细胞为Thy-1-，B220+，一个亚群为sIgM+。胸腺内B细胞具有成人胸腺B细胞的特征，包括CD 5表达和对IL-4 + IL-5 + CD 40配体的增殖反应，但不对LPS或可溶性抗IgM反应。在用IL-4 + IL-5 + LPS和IL-4 + IL-5 + CD 40配体刺激后观察到IG产生。总之，我们已经证明flt 3L + IL-7支持胎儿胸腺中存在的祖细胞亚群的扩增。培养的祖细胞可以重新填充胎儿胸腺叶并发育成成熟的功能性B细胞，表明胎儿胸腺能够支持B细胞以及T细胞发育。
Flt3 ligand (flt3L) has potent effects on hemopoietic progenitors, dendritic cells, and B lymphopoiesis. We have investigated the effects of flt3L on intrathymic precursors. The addition of flt3L + IL-7 to lobe submersion cultures of murine fetal thymic lobes resulted in the expansion of an immature population of Thy-1(low), CD44(high), HSA(high) cells. This population contained cells with precursor activity, as determined by their capacity to repopulate deoxyguanosine-treated fetal thymic lobes. Upon reentry to the thymic lobe, flt3L + IL-7-cultured Thy-1(low), CD44(high), HSA(high) cells underwent expansion and differentiation into B cells. Two weeks after fetal thymic organ culture following thymic lobe reconstitution, intrathymic cells were Thy-1-, B220+, and a subset was sIgM+. The intrathymic B cells shared features of adult thymic B cells, including CD5 expression and proliferative responses to IL-4 + IL-5 + CD40 ligand, but not to LPS or soluble anti-IgM. Ig production was noted upon stimulation with IL-4 + IL-5 + LPS and IL-4 + IL-5 + CD40 ligand. In conclusion, we have demonstrated that flt3L + IL-7 supports the expansion of a subset of progenitors present in the fetal thymus. The cultured progenitors can repopulate a fetal thymic lobe and develop into mature functional B cells, demonstrating that the fetal thymus is able to support B cell as well as T cell development.
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胸腺基质细胞系支持表面 IgM B 细胞的体外发育，并产生影响 B 和 T 谱系细胞的新型生长因子。
影响因子：
2.6
作者：
Friend,SL;Hosier,S;Nelson,A;Foxworthe,D;Williams,DE;Farr,A
通讯作者：
Farr,A
使用表达胸苷激酶自杀基因的供体 T 细胞调节骨髓移植后的同种异体反应。
影响因子：
2.6
作者：
Drobyski,WilliamR;Gendelman,Maria
通讯作者：
Gendelman,Maria
影响因子：
32.4
作者：
MACKAREHTSCHIAN, K;HARDIN, JD;LEMISCHKA, IR
通讯作者：
LEMISCHKA, IR
胸腺基质细胞可以支持 B 细胞从胸腺内前体细胞分化。
DOI：
--
发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Montecino-Rodriguez,E;Johnson,A;Dorshkind,K
通讯作者：
Dorshkind,K