Measurement of neopterin, TGF-β1 and ACE in the exhaled breath condensate of patients with sarcoidosis
Measurement of neopterin, TGF-β1 and ACE in the exhaled breath condensate of patients with sarcoidosis
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DOI：
10.1088/1752-7155/7/4/046003
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发表时间：
2013-12-01
影响因子：
3.8
通讯作者：
Thomas, Paul S.
中科院分区：
文献类型：
--
作者：
Ahmadzai, Hasib;Cameron, Barbara;Thomas, Paul S.
呼出的呼吸冷凝液(EBC)是一种非侵入性的呼吸道疾病呼吸道衬里液体采样方法。这可能有助于确定肉芽肿性炎症和结节病患者肺纤维化的呼出生物标志物。这项初步研究的目的是确定肉芽肿性呼吸道炎症和疾病活动性的标志，包括EBC中的新喋呤、转化生长因子-β(1)和血管紧张素转换酶(ACE)。EBC来自16例结节病患者和22例健康对照。采用双抗体夹心法测定EBC、新喋呤和活性转化生长因子-β(1)的含量。用比色法测定EBC-ACE活性。EBC新喋呤在3/20名对照组和7/16名结节病患者中检测到。结节病患者的平均新喋呤水平高于对照组(0.57+/-0.45nmol L(-1)比0.41+/-0.22nmol L(-1)，P=0.04)。所有受试者外周血中均可检测到转化生长因子-β(1)，结节病患者外周血中转化生长因子-β(1)浓度高于对照组(115.5±79.6pgmol(-1)比82.3+/-16.2pgmol(-1，p=0.048)。EBC血管紧张素转换酶活性无明显差异，仅在3/20例健康人和2/16例患者中检测到(P=0.91)。肉芽肿性炎症的EBC标记物在结节病患者中的检测水平比健康对照组更高。有必要进行更大规模的研究和开发灵敏的分析方法，以检查这些标记物的疾病相关性和预测效用。
Exhaled breath condensate (EBC) is a non-invasive method of sampling airway lining fluids in respiratory diseases. This may be useful in identifying exhaled biomarkers of granulomatous inflammation and pulmonary fibrosis in patients with sarcoidosis. The aim of this pilot study was to identify markers of granulomatous airway inflammation and disease activity including neopterin, transforming growth factor-beta(1) (TGF-beta(1)) and angiotensin converting enzyme (ACE) in EBC. EBC was collected from 16 patients with sarcoidosis and 22 healthy control subjects. EBC neopterin, and active-TGF-beta(1) were measured by ELISA. EBC-ACE activity was measured using a colorimetric assay. EBC neopterin was detectable in 3/20 controls and 7/16 patients with sarcoidosis. Patients with sarcoidosis had greater mean neopterin levels compared to control subjects (0.57 +/- 0.45 nmol l(-1) versus 0.41 +/- 0.22 nmol l(-1), p = 0.04). TGF-beta(1) was detectable in the EBC of all subjects and concentrations were higher in patients with sarcoidosis compared with controls (115.5 +/- 79.6 pg mol(-1) versus 82.3 +/- 16.2 pg mol(-1), p = 0.048). There was no difference in EBC ACE activity, which was only detectable in 3/20 healthy controls and 2/16 patients (p = 0.91). EBC markers of granulomatous inflammation are detectable at greater levels in patients with sarcoidosis compared to healthy controls subjects. Larger studies and development of sensitive assays are warranted to examine the disease correlates and predictive utility of these markers.