A carotenoid-binding protein (CBP) plays a crucial role in cocoon pigmentation of silkworm (Bombyx mori) larvae
A carotenoid-binding protein (CBP) plays a crucial role in cocoon pigmentation of silkworm (Bombyx mori) larvae
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DOI：
10.1016/j.febslet.2004.04.067
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发表时间：
2004-06-04
期刊：
影响因子：
3.5
通讯作者：
Sato, R
中科院分区：
文献类型：
--
作者：
Tabunoki, H;Higurashi, S;Sato, R
我们研究了类胡萝卜素结合蛋白（CBP）在黄茧色素沉着中的作用。首先，使用黄色或白色茧品种，我们研究了黄色色素沉着和CBP表达之间的联系。CBP仅在黄茧品种的丝腺中表达，黄茧品种利用类胡萝卜素进行茧着色。此外，CBP在第1-7天的5龄幼虫的丝腺中的表达与类胡萝卜素吸收到丝腺中的时期相匹配。最后，我们将双链CBP RNA导入家蚕（B.桑（mori）幼虫诱导RNA干扰。在第4天证实了CBP在五龄幼虫丝腺中的表达显著降低，并且观察到茧中的黄色色素减少。我们发现CBP在类胡萝卜素引起的黄茧色素沉着中起关键作用。(C)2604欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
We examined the role of carotenoid-binding protein (CBP) in yellow cocoon pigmentation. First, using yellow or white cocoon races, we investigated the linkage between the yellow pigmentation and CBP expression. CBP was expressed only in the silk gland of the yellow cocoon races, which utilize carotenoids for cocoon pigmentation. Furthermore, CBP expression in the silk glands of day 1-7 fifth instar larvae matched the period of carotenoid uptake into the silk gland. Finally, we gave double-stranded CBP RNA to Bombyx mori (B. mori) larvae to induce RNA interference. The significantly reduced expression of CBP in the silk gland of fifth instar larva was confirmed on day 4 and a decrease in yellow pigmentation was observed in the cocoon. We showed that CBP plays a key role in the yellow cocoon pigmentation caused by carotenoids. (C) 2604 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.