The anatomy of mammalian sweet taste receptors
The anatomy of mammalian sweet taste receptors
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DOI：
10.1002/prot.25228
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发表时间：
2017-02-01
影响因子：
2.9
通讯作者：
Fiorucci, Sebastien
中科院分区：
文献类型：
--
作者：
Cheron, Jean-Baptiste;Golebiowski, Jerome;Fiorucci, Sebastien
所有的甜味化合物都是由一个单一的G蛋白偶联受体（GPCR），异二聚体T1 R2-T1 R3检测，没有实验结构。甜味受体是C类GPCR，最近发表的代谢型谷氨酸受体（mGluR）1和5的晶体结构为理解该家族内的结构-功能关系提供了重要的一步。在这篇文章中，我们概括了600多个单点定点突变和可用的结构数据，以获得甜味受体序列与其他C类GPCR的关键比对。使用这种对齐，一个同源的3D模型的人类甜味受体的建立和分析，解剖出参与配体结合的关键残基和那些负责受体激活的作用。蛋白质2017; 85：332-341。(c)2016 Wiley Periodicals，Inc.
All sweet-tasting compounds are detected by a single G-protein coupled receptor (GPCR), the heterodimer T1R2-T1R3, for which no experimental structure is available. The sweet taste receptor is a class C GPCR, and the recently published crystallographic structures of metabotropic glutamate receptor (mGluR) 1 and 5 provide a significant step forward for understanding structure-function relationships within this family. In this article, we recapitulate more than 600 single point site-directed mutations and available structural data to obtain a critical alignment of the sweet taste receptor sequences with respect to other class C GPCRs. Using this alignment, a homology 3D-model of the human sweet taste receptor is built and analyzed to dissect out the role of key residues involved in ligand binding and those responsible for receptor activation. Proteins 2017; 85:332-341. (c) 2016 Wiley Periodicals, Inc.