Detection of specific and 'constitutive' antibody secreting cells in the gills, head kidney and peripheral blood leucocytes of dab (Limanda limanda)
Detection of specific and 'constitutive' antibody secreting cells in the gills, head kidney and peripheral blood leucocytes of dab (Limanda limanda)
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DOI：
10.1016/s0165-2427(97)00017-2
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发表时间：
1997-09-19
影响因子：
1.8
通讯作者：
Ellis, AE
中科院分区：
文献类型：
--
作者：
Davidson, GA;Lin, SH;Ellis, AE
采用ELISPOT法，通过对淡水鱼（Limanda limanda）鳃、头肾和血液中抗体分泌细胞（ASC）的计数，研究了鳃在抗体产生方面的相对免疫学重要性。鳃中“组成型”ASC的贡献似乎比引发的特定ASC更大。发现鳃含有4227 +/- 1029“组成型”ASC/10（6）细胞的平均值（+/-SD），这少于头肾含有15617 +/- 3723“组成型”ASC/10（6）细胞的平均值（+/-SD），但多于外周血白细胞，其含有2650 +/- SD。212个“组成型”ASC/10（6）细胞。还测定了HGG胃肠外或口服给药后特异性抗人γ球蛋白（HGG）ASC的数量。在胃肠外免疫后，在所有三种组织中检测到抗HGG ASC，在免疫后4周同时达到峰值。在头肾中发现最强的反应。口服免疫后，反应弱得多;再次头肾是最活跃的，但鳃反应几乎检测不到。通过ELISA测定血清中的特异性抗体补充了这些数据。免疫后的血清抗体滴度被发现密切相关的具体ASC在头肾后胃肠外免疫，而口服抗原后的血清抗体滴度最密切的后续的具体ASC在血液中的数量。根据这些数据，它表明，在鳃中的白细胞的主要免疫作用可能是在最早的阶段防御感染。(C)1997年Elsevier Science B.V.
The relative immunological importance of the gills of fish was investigated in terms of antibody production by enumerating antibody secreting cells (ASC) in the gills, head kidney and blood of dab (Limanda limanda) using the ELISPOT assay. The contribution of 'constitutive' ASC in the gill appeared more substantial than that of elicited specific ASC. The gills were found to contain a mean (+/-SD) of 4227 +/- 1029 'constitutive' ASC/10(6) cells which was fewer than the head kidney which contained a mean (+/-SD) of 15617 +/- 3723 'constitutive' ASC/10(6) cells but more than peripheral blood leucocytes which contained a mean (+/- SD) of 2650 +/- 212 'constitutive' ASC/10(6) cells. The number of specific anti-human gamma globulin (HGG) ASC following parenteral or oral administration of HGG was also determined. Anti-HGG ASC were detected in all three tissues following parenteral immunization, peaking simultaneously, 4 weeks post-immunization. The strongest response was found in the head kidney. After oral immunization, responses were much weaker; again the head kidney was the most active but the gill response was barely detectable. These data were complemented by measurement of specific antibody in the serum by ELISA. Serum antibody titres following immunization were found to correlate closely with the number of specific ASC in the head kidney following parenteral immunization whereas serum antibody titres after oral administration of antigen most closely followed the number of specific ASC in the blood In the Light of these data it is suggested that the primary immunological role of the leucocytes in the gill may be in the earliest stages of defence against infection. (C) 1997 Elsevier Science B.V.