Digestive Stability of Xanthophylls Exceeds That of Carotenes As Studied in a Dynamic in Vitro Gastrointestinal System
Digestive Stability of Xanthophylls Exceeds That of Carotenes As Studied in a Dynamic in Vitro Gastrointestinal System
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DOI：
10.3945/jn.108.103655
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发表时间：
2009-05-01
影响因子：
4.2
通讯作者：
Alric, Monique
中科院分区：
文献类型：
--
作者：
Blanquet-Diot, Stephanie;Soufi, Maha;Alric, Monique
流行病学研究表明，番茄制品的高消费量与慢性疾病的风险较低有关。为了发挥其健康作用，番茄的植物化学物质必须是生物可利用的，因此这意味着它们在消化过程中的稳定性。在这里，我们评估的消化稳定性的红色色素的番茄红素和其他类胡萝卜素带来的营养量在不同的食物基质，使用TNO胃肠道模型（TIM）。该多室动力学系统准确地再现了人体胃和小肠消化的主要参数。对含有红番茄（RT）、黄番茄（缺乏番茄红素）或番茄红素珠粒的标准膳食进行体外消化。玉米黄质和叶黄素是稳定的整个人工digestion，而β-胡萝卜素和全反式番茄红素降解（类似30和20%的损失，在消化结束时，分别）在空肠和回肠隔室。在RT餐的面包中β-胡萝卜素的回收率显著低于黄色的面包，显示出食物基质效应。以同样的方式，直到180分钟的消化，从RT的全反式番茄红素的回收率显着低于那些发出的补充。异构体构象也影响类胡萝卜素的稳定性，5-顺式番茄红素是最稳定的异构体，其次是全反式和9-顺式。在TIM中没有发生番茄红素的反-顺异构化。通过使用相关的动态体外系统，这项研究使我们能够进一步了解影响类胡萝卜素的消化稳定性的参数，因此，它们的生物利用度，在人类。J.营养139：876-883，2009.
Epidemiological studies have suggested that high consumption of tomato products is associated with a lower risk for chronic diseases. To exert their health effect, the phytochemicals of tomatoes have to be bioavailable and therefore it implies their stability through the digestion process. Here, we assessed the digestive stability of the red-pigmented lycopene and other carotenoids brought in nutritional quantity within different food matrixes, using the TNO gastrointestinal tract model (TIM). This multicompartmental dynamic system accurately reproduces the main parameters of gastric and small intestinal digestion in human. In vitro digestions of a standard meal containing red tomato (RT), yellow tomato (devoid of lycopene), or lycopene beadlets were performed. Zeaxanthin and lutein were stable throughout artificial digestions, whereas beta-carotene and all-trans lycopene were degraded (similar to 30 and 20% loss at the end of digestion, respectively) in the jejunal and ileal compartments. The recovery of beta-carotene in the digesta of the RT meal was significantly lower than that in the yellow one, showing a food matrix effect. In the same way, until 180 min of digestion, the recovery percentages of all-trans lycopene from RT were significantly lower than those issued from the supplement. Isomeric conformation also influenced the stability of carotenoids, 5-cis lycopene being the most stable isomer followed by all-trans and 9-cis. No trans-cis isomerization of lycopene occurred in the TIM. By using a relevant dynamic in vitro system, this study allowed us to gain further insight into the parameters influencing the digestive stability of carotenoids, and therefore their bioavailability, in humans. J. Nutr. 139: 876-883, 2009.