Artifactual synchrony via capacitance coupling in multi-electrode recording from cat striate cortex.
Artifactual synchrony via capacitance coupling in multi-electrode recording from cat striate cortex.
复制标题
通过猫纹状皮层多电极记录中的电容耦合实现人工同步。
DOI：
10.1016/s0165-0270(01)00529-5
复制
发表时间：
2002
影响因子：
3
通讯作者：
Kasamatsu,Takuji
中科院分区：
文献类型：
--
作者：
Zhu,Zhao;Lin,Kevin;Kasamatsu,Takuji
对大脑中神经连接模式的阐释被认为能让我们对大脑如何工作有一个机械的理解。功能连接最好通过同时记录多个神经元的单个活动来研究。因此，各种类型的多微电极系统被开发出来。我们研究了猫纹状皮层的远距离横向相互作用。为了从生理上表征同时记录的相互作用细胞，我们使用了两个微电极，其运动由两个独立可移动的微驱动器控制。两个微电极的尖端间距为~ 2mm或更多。在初步绘制细胞对的两个感受野时，我们经常注意到两个微电极记录的两个尖峰序列（振幅比约为20:1）之间出现完全同步的放电。同步放电时，较小的尖峰消失，当较大的尖峰丢失时，要么是实质性的推进，要么是在电极上放置电解损伤，记录后者。当两个微驱动系统被单独屏蔽时，同步也消失了。我们认为同步是通过两个微驱动系统之间的电容耦合实现的。我们提出了一些实用的建议，以避免交叉相关图的污染与假阳性，窄峰在零时间由于反射峰列的存在。
Elucidation of neural connectivity patterns in the brain are thought to give us a mechanistic understanding of how the brain works. Functional connectivity is best studied by simultaneous recording of single-unit activity from many neurons. Accordingly, various types of multiple-microelectrode systems have been developed. We have studied long-range lateral interactions in cat striate cortex. To physiologically characterize interacting cells recorded simultaneously, we used two microelectrodes whose movements were controlled by two independently-movable microdrives. The tips of the two microelectrodes were separated by ∼2 mm or more. During preliminary plotting of two receptive fields of cell pairs, we often noted the emergence of perfectly synchronous firing between two spike trains (amplitude ratio, about 20:1) registered with two microelectrodes. Synchronously firing, smaller spikes disappeared when larger spikes of the pair were lost to either substantial advancement of or placing an electrolytic lesion at the electrode registering the latter. The synchrony also disappeared when two microdrive systems were shielded individually. We concluded that the synchrony was attained through capacitance coupling between two microdrive systems. We proposed a few practical recommendations to avoid the contamination of cross correlograms with the false-positive, narrow peak at time zero due to the presence of reflected spike trains.