Activation of divalent cation influx into S. cerevisiae cells by hypotonic downshift.
Activation of divalent cation influx into S. cerevisiae cells by hypotonic downshift.
复制标题
通过低渗降移激活二价阳离子流入酿酒酵母细胞。
DOI：
10.1007/s002329900296
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Dunn,T
中科院分区：
文献类型：
--
作者：
Beeler,T;Gable,K;Dunn,T
通过将细胞从含有0.8山梨糖醇的YPD培养基转移到不含山梨糖醇的YPD培养基中，使酿酒酵母细胞处于低渗下移状态，可诱导Ca2+和其他二价阳离子瞬间快速流入细胞。对于在37°C的YPD中生长的细胞，这种低压下移使Ca2+积累增加6.7倍。十二胺和Mg2+对等渗YPD培养基中低渗降速诱导的Ca2+积累和稳态Ca2+积累有不同的影响。Ca2+内流通路负责低渗诱导的Ca2+内流，可能占YPD中生长的细胞Ca2+积累的约10-35%。Ca2+内流不是细胞在低渗降移中存活所必需的。低渗降频大大降低了s。细胞在暴露于10 mmCd2+ 5分钟后存活，这表明抗急性Cd2+暴露的突变体可能有助于识别低渗下行诱导的二价阳离子内流所需的基因。
SubjectingSaccharomyces cerevisiaecells to a hypotonic downshift by transferring cells from YPD medium containing 0.8msorbitol to YPD medium without sorbitol induces a transient rapid influx of Ca2+and other divalent cations into the cell. For cells grown in YPD at 37°C, this hypotonic downshift increases Ca2+accumulation 6.7-fold. Hypotonic downshift-induced Ca2+accumulation and steady-state Ca2+accumulation in isotonic YPD medium are differentially affected by dodecylamine and Mg2+. The Ca2+-influx pathway responsible for hypotonic-induced Ca2+influx may account for about 10–35% of Ca2+accumulation by cells growing in YPD. Ca2+influx is not required for cells to survive a hypotonic downshift. Hypotonic downshift greatly reduces the ability ofS. cerevisiaecells to survive a 5-min exposure to 10 mmCd2+suggesting that mutants resistant to acute Cd2+exposure may help identify genes required for hypotonic downshift-induced divalent cation influx.