Genome wide cDNA-AFLP analysis of genes rapidly induced by combined sucrose and ABA treatment in rice cultured cells

Genome wide cDNA-AFLP analysis of genes rapidly induced by combined sucrose and ABA treatment in rice cultured cells
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DOI:
10.1016/j.febslet.2006.09.065
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发表时间:
2006-10-30
期刊:
影响因子:
3.5
通讯作者:
Fujimura, Tatsuhito
Fujimura, Tatsuhito
中科院分区:
生物学3区
文献类型:
--
作者:
Akihiro, Takashi;Umezawa, Taishi;Fujimura, Tatsuhito

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利用cDNA-AFLP技术从水稻培养细胞中鉴定了27个蔗糖和阿坝联合处理诱导表达的基因。共处理后30分钟,这些上调的基因中有13个被诱导。这套基因包括淀粉生物合成相关基因。A型基因仅在蔗糖和阿坝存在下表达。B型基因在蔗糖或阿坝存在下表达,并且通过蔗糖和阿坝的共处理显著增强表达。这些结果表明,淀粉生物合成和其他过程的多个步骤可能受到至少两种不同途径的调节。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版,保留所有权利。
We identified 27 genes induced by combined sucrose and ABA treatment from rice cultured cells with cDNA-AFLP. Thirteen of these up-regulated genes were induced 30 min after the co-treatment. This suite of genes includes starch biosynthesis related genes. Type A genes were expressed only in the presence of both sucrose and ABA. Type B genes were expressed in the presence of sucrose or ABA and the expression was dramatically enhanced by the co-treatment of sucrose and ABA. These results indicate that multiple steps of starch biosynthesis and other processes may be regulated by at least two different pathways. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.