Sequence analysis of VP2 gene of canine parvovirus isolated from domestic dogs in Japan in 1999 and 2000
Sequence analysis of VP2 gene of canine parvovirus isolated from domestic dogs in Japan in 1999 and 2000
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DOI：
10.1016/j.cimid.2006.05.001
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发表时间：
2006-07-01
影响因子：
2
通讯作者：
Kai, Chieko
中科院分区：
文献类型：
--
作者：
Doki, Miho;Fujita, Kentaro;Kai, Chieko
从1999 ~ 2000年日本犬细小病毒（CPV）病犬中分离到7株CPV，并对其VP 2基因进行了遗传分析。VP 2氨基酸序列预测结果表明，3株为CPV 2a型，4株为CPV 2b型。从VP 2基因构建的系统发育树表明，新分离的毒株被归类到最近的日本和台湾的现场分离株，这是不同于越南分离株，美国分离株，或经典的CPV 2型组成的集群。这些结果表明，CPV在20世纪90年代在日本和台湾之间发生了传播，并且后代仍然在两国传播。(c)2006爱思唯尔有限公司保留所有权利。
Seven strains of canine parvovirus (CPV) were isolated from affected dogs in Japan between 1999 and 2000, and their VP2 genes were genetically analyzed. Comparison of the predicted amino acid sequences of VP2 suggested that three field isolates corresponded to CPV type 2a, while the other four to CPV type 2b. The phylogenetic tree constructed from the VP2 genes showed that the newly isolated strains are classified into the cluster consisting of recent Japanese and Taiwanese field isolates, which are distinct from Vietnamese isolates, United States Isolates, or classical CPV type 2. These results suggest that the CPV transmission occurred between Japan and Taiwan in 1990s, and the offspring are still circulating in both countries. (c) 2006 Elsevier Ltd. All rights reserved.