Definition of germinal-center B cell migration in vivo reveals predominant intrazonal circulation patterns
Definition of germinal-center B cell migration in vivo reveals predominant intrazonal circulation patterns
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DOI：
10.1016/j.immuni.2007.04.008
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发表时间：
2007-05-01
期刊：
影响因子：
32.4
通讯作者：
Haberman, Ann M.
中科院分区：
文献类型：
--
作者：
Hauser, Anja E.;Junt, Tobias;Haberman, Ann M.
生殖中心（GC）的增殖、突变和选择被认为发生在不同的微解剖区室-暗区（DZ）和亮区（LZ）。因此，亲和力成熟被认为需要在区域之间频繁的运输。在这里，我们报告使用多光子在体内显微镜，以确定迁移模式的GC B细胞。时间分辨图像的分析揭示了意想不到的运动模式以及GC B细胞形态。尽管预期DZ和LZ之间会频繁移动，但观察到很少细胞穿过两个隔室之间的界面。此外，细胞轨迹表明，在这个区域的细胞运动主要是平行的接口，这表明B细胞循环内的个别LZ和DZ室。结果表明，我们的观点的B细胞循环GC内的功能关系，它的两个主要车厢的修订。
Proliferation, mutation, and selection in the germinal center (GC) are thought to occur in distinct microanatomical compartments-the dark zone (DZ) and the light zone (LZ). Thus, affinity maturation has been posited to require frequent trafficking between zones. Here we report the use of multiphoton in vivo microscopy to determine migration patterns of GC B cells. Analysis of time-resolved images revealed unexpected patterns of movement as well as GC B cell morphology. Though frequent movement between the DZ and LZ was anticipated, few cells were observed to cross the interface between the two compartments. Moreover, cell-track trajectories indicated that cell movement in this region is predominantly parallel to the interface, suggesting that B cells circulate within individual LZ and DZ compartments. The results suggest a revision to our views of B cell circulation within GCs and the functional relationship of its two major compartments.