OsCRS2encoding a peptidyl-tRNA hydrolase protein is essential for chloroplast development in rice
OsCRS2encoding a peptidyl-tRNA hydrolase protein is essential for chloroplast development in rice
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OsCRS2编码肽基-tRNA水解酶蛋白对于水稻叶绿体发育至关重要
DOI：
10.1007/s10725-020-00655-8
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发表时间：
2020
影响因子：
4.2
通讯作者：
Qian Qian
中科院分区：
文献类型：
--
作者：
Zhang Qiang;Wang Yaliang;Shen Lan;Ren Deyong;Hu Jiang;Zhu Li;Zhang Guangheng;Guo Longbiao;Zeng Dali;Qian Qian
叶绿体被认为是光合作用、生物合成和代谢产物储存的重要细胞器。到目前为止，叶绿体发育的分子调控机制还不清楚。叶绿体RNA剪接2(CRS2)蛋白影响玉米叶绿体基因内含子的剪接，在叶绿体发育过程中起关键作用。然而，OsCRS2在水稻叶绿体发育中的作用还鲜有研究。本研究利用CRISPR/Cas9基因编辑技术获得了oscrs2-1和oscrs2-2突变体，用于基因功能研究。两个突变体均表现为白化苗表型。OsCRS2位于叶绿体中，在绿色组织中高表达。OsCRS2可影响第一组和第二组7个内含子的剪接，并参与叶绿体rRNA的生物发生。RNA测序分析表明，在Theoscrs2-1突变体中，核编码的光合作用和叶绿体发育相关基因的表达都显著降低。综上所述，这些结果表明OsCRS2是水稻叶绿体早期发育所必需的。
Chloroplasts are identified as important organelles for photosynthesis, biosynthesis, and storage of metabolites. So far, the molecular regulatory mechanism of chloroplast development remains to be unclear. The chloroplast RNA splicing 2 (CRS2) protein was determined to play a key role in chloroplast development as they affect the splicing of chloroplast gene introns in maize. However, the functions ofOsCRS2in rice chloroplast development have rarely been studied. In this study,oscrs2-1andoscrs2-2mutants were obtained to gene function research using the CRISPR/Cas9 gene editing technology. Both of the mutants displayed an albino seedling phenotype. The OsCRS2 is located in the chloroplast, and is highly expressed in green tissues. OsCRS2 could affect group I and seven group II introns splicing and participate in chloroplast rRNA biogenesis. RNA sequencing analysis indicated that both nuclear-encoded photosynthesis and chloroplast development-related genes expression were significantly reduced in theoscrs2-1mutant. To conclude, these results suggested that OsCRS2 is necessary for early chloroplast development in rice.