Functional and molecular changes in the photosynthetic apparatus during senescence of flag leaves from field-grown barley plants
Functional and molecular changes in the photosynthetic apparatus during senescence of flag leaves from field-grown barley plants
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DOI：
10.1111/j.1365-3040.1996.tb00256.x
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发表时间：
1996-03-01
影响因子：
7.3
通讯作者：
Krupinska, K
中科院分区：
文献类型：
--
作者：
Humbeck, K;Quast, S;Krupinska, K
本文分析了大麦成熟衰老过程中光合机构功能和组成的变化。在田间条件下，旗叶在开花后8d左右，光合能力（以光饱和条件下的氧释放量测量）和D-1蛋白水平开始下降，在开花后14d左右，色素含量、光系统II效率（F-v/F-m）和细胞色素f和核酮糖-1，5-二磷酸羧化酶大亚基水平开始下降，细胞色素b559和核酮糖-1，5-二磷酸羧化酶的小亚基的水平在开花后22 d左右下降，在衰老后期仍然可以检测到高水平的光系统IT捕光复合物，在花后26 d，编码光合机构成分的转录本水平下降的动力学也存在差异，rbcS mRNA丰度在花后4 d左右剑叶达到其最终长度时已经下降，而psbA和petC转录物水平直到花后16 d才下降。相反，衰老相关转录物，pHvS40（Becker & Apel 1993），描述了花后14 d旗叶中积累的几种不同的衰老过程，讨论了这些过程之间的相互关系及其调控。
Changes in the function and composition of the photosynthetic apparatus were analysed during maturation and senescence of barley (Hordeum vulgare L.) flag leaves under field conditions, At about 8 d post-anthesis, photosynthetic capacity, measured as oxygen evolution under light-saturating conditions, and the D-1 protein level started to decrease, At about 14 d post-anthesis, the pigment content, photosystem II efficiency (F-v/F-m) and levels of cytochrome f and the large subunit of ribulose-1,5-bisphosphate carboxylase started to decline, The levels of cytochrome b559 and the small subunit of ribulose-1,5-bisphosphate carboxylase decreased later, at about 22 d post-anthesis, High levels of the light-harvesting complex of photosystem IT were still detectable at an even later stage of senescence, at 26 d post-anthesis.There were also differences in the kinetics of the declines in the levels of transcripts coding for components of the photosynthetic apparatus, rbcS mRNA abundance had already decreased when flag leaves reached their final lengths, at about 4 d post-anthesis, whereas levels of psbA and petC transcripts did not decrease until 16 d post-anthesis, In contrast, a senescence-related transcript, pHvS40 (Becker & Apel 1993), accumulated in the flag leaves 14 d post-anthesis.Several senescence-related processes which differ in their kinetics are described, The interrelationships among these processes and their regulation are discussed.