CELL-DEATH IN THE DEVELOPING TRIGEMINAL NUCLEAR-COMPLEX OF THE RAT
CELL-DEATH IN THE DEVELOPING TRIGEMINAL NUCLEAR-COMPLEX OF THE RAT
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DOI：
10.1016/0165-3806(91)90089-2
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发表时间：
1991-11-19
期刊：
影响因子：
--
通讯作者：
WAITE, PME
中科院分区：
文献类型：
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作者：
ASHWELL, KWS;WAITE, PME
借助于尼氏物质和琥珀酸脱氢酶活性染色的切片，检查了大鼠三叉神经复合体中细胞死亡的时间过程和分布。 对三叉神经主核和三个脊髓三叉神经亚核中的每一个的固缩图计数显示，细胞死亡始于 E19，位于主核和口亚核之间的连接区域，靠近三叉神经传入进入脑干的部位。 细胞死亡随后向头侧和尾侧扩散到其余的主核和脊髓三叉神经核中。 在检查的三叉神经复合体的所有部分中，大约在 P10 时，同时出现细胞死亡病例。 无法将神经元与产前动物中的神经胶质细胞可靠地区分开来，但出生后的神经元数量数据表明，大部分细胞死亡确实是由于神经元损失所致。 数据表明，在三叉神经核复合体中，只有一半的神经元能够存活到成熟。 这些发现对于那些使用该系统进行可塑性研究的研究人员具有重要意义。
The time course and distribution of cell death in the trigeminal nuclear complex of the rat has been examined with the aid of sections stained for Nissl substance and succinic dehydrogenase activity. Pyknotic figure counts in the principal trigeminal nucleus and in each of the three spinal trigeminal subnuclei revealed that cell death commences at E19, in the region of the junction between the principal nucleus and the subnucleus oralis, close to the site of entry of trigeminal afferents into the brainstem. Cell death subsequently spreads rostrally and caudally into the rest of the principal and spinal trigeminal nuclei. Cell death cases simultaneously, at about P10, in all parts of the trigeminal nuclear complex examined. Neurons could not be reliably distinguished from glial cells in prenatal animals, but data for neuronal numbers postnatally indicate that much of this cell death is indeed due to loss of neurons. The data suggest that, in the trigeminal nuclear complex, only half the number of neurons produced survive to maturity. These findings are of significance for those investigatiors using this system in studies of plasticity.