Distinct regional patterns in noradrenergic innervation of the rat prefrontal cortex
Distinct regional patterns in noradrenergic innervation of the rat prefrontal cortex
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DOI：
10.1016/j.jchemneu.2019.01.002
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发表时间：
2019-03-01
影响因子：
2.8
通讯作者：
Coutureau, Etienne
中科院分区：
文献类型：
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作者：
Cerpa, Juan-Carlos;Marchand, Alain R.;Coutureau, Etienne
啮齿类动物前额叶皮层的解剖和功能已被广泛研究。现在很清楚，PFC是各种执行功能的核心，这些功能依赖于单胺能神经调节。PFC接受来自单胺能核团的广泛投射，特别是来自蓝斑（LC）的投射，蓝斑是皮质中去甲肾上腺素（NA）的主要来源。这个核团的投射长期以来被认为是在整个大脑中扩散和均匀地发挥作用。然而，最近的研究表明，一个单独的神经支配的前额叶子区域的非侧支LC神经元，这表明其功能的特定调制。根据这一想法，我们的目的是更准确地描述去甲肾上腺素能神经支配到不同的眶（OFC）和内侧（mPFC）的亚区的模式。我们集中在外侧（LO），腹侧（VO）和内侧（MO）的OFC的部分，和在mPFC的32 d（A32 d），32 v（A32 v）和25（A25）区。使用多巴胺-β-羟化酶作为特异性去甲肾上腺素能标记物，我们对这些子区域的去甲肾上腺素能纤维和静脉曲张进行了自动定量。结果表明，去甲肾上腺素能神经支配在沿着吻尾轴的某些前额叶亚区是不均匀的。功能分离最近已被报道，在前额叶子区域沿着喙尾方向。我们的研究结果为这一新兴想法提供了神经解剖学支持。
The anatomy and functions of the rodent prefrontal cortex (PFC) have been extensively studied. It is now clear that the PFC is at the core of various executive functions and that these functions depend on monoaminergic neuromodulation. The PFC receives extensive projections from monoaminergic nuclei and, in particular, from the locus coeruleus (LC) which is the major source of noradrenaline (NA) in the cortex. Projections of this nucleus have long been considered to act diffusely and uniformly throughout the entire brain. However, recent studies have revealed a separate innervation of prefrontal sub-regions by non-collateralizing LC neurons, suggesting a specific modulation of their functions. Following this idea, we aimed at describing more precisely the pattern of noradrenergic innervation into different orbital (OFC) and medial (mPFC) sub-regions of the PFC. We focused on the lateral (LO), ventral (VO) and medial (MO) portions of the OFC, and on areas 32d (A32d), 32v (A32v) and 25 (A25) in the mPFC. Using Dopamine-beta-Hydroxylase as a specific noradrenergic marker, we performed an automatic quantification of noradrenergic fibers and varicosities in each of these sub-regions. The results indicate that noradrenergic innervation is heterogeneous in some prefrontal sub-regions along the rostro-caudal axis. Functional dissociations have been recently reported in prefrontal sub-regions along the rostro-caudal direction. Our findings add neuroanatomical support to this emergent idea.