Aerosol capture and coronavirus spike protein deactivation by enzyme functionalized antiviral membranes.
Aerosol capture and coronavirus spike protein deactivation by enzyme functionalized antiviral membranes.
复制标题
通过酶功能化抗病毒膜捕获气溶胶和使冠状病毒刺突蛋白失活。
DOI：
10.1038/s43246-022-00256-0
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发表时间：
2022
影响因子：
7.8
通讯作者：
Bhattacharyya,Dibakar
中科院分区：
文献类型：
--
作者：
Mills,Rollie;Vogler,RonaldJ;Bernard,Matthew;Concolino,Jacob;Hersh,LouisB;Wei,Yinan;Hastings,JeffreyTodd;Dziubla,Thomas;Baldridge,KevinC;Bhattacharyya,Dibakar
冠状病毒传播的空气传播特性使开发能够同时减少气溶胶和病毒传播的新屏障技术变得至关重要。在这里，我们报告了厚度和孔隙率可调的纳米结构膜用于过滤冠状病毒大小的气雾剂，结合抗病毒酶功能化可以在低水合环境中变性SARS-CoV-2病毒的尖峰糖蛋白。具有枯草杆菌酶和甲基丙烯酸官能化的不对称薄膜对100 nm非官能化和蛋白质官能化的聚苯乙烯乳胶气溶胶粒子的过滤效率超过98.90%。非功能化膜对冠状病毒大小的颗粒提供了540 ± 380的保护因子，高于职业安全与健康管理局对N95口罩的10标准。用表面含水率为0.02~0.2%的枯草杆菌酶功能化膜在30 的S溶液中变性冠状病毒颗粒表面的SARS-CoV-2刺激性糖蛋白。
The airborne nature of coronavirus transmission makes it critical to develop new barrier technologies that can simultaneously reduce aerosol and viral spread. Here, we report nanostructured membranes with tunable thickness and porosity for filtering coronavirus-sized aerosols, combined with antiviral enzyme functionalization that can denature spike glycoproteins of the SARS-CoV-2 virus in low-hydration environments. Thin, asymmetric membranes with subtilisin enzyme and methacrylic functionalization show more than 98.90% filtration efficiency for 100-nm unfunctionalized and protein-functionalized polystyrene latex aerosol particles. Unfunctionalized membranes provided a protection factor of 540 ± 380 for coronavirus-sized particle, above the Occupational Safety and Health Administration’s standard of 10 for N95 masks. SARS-CoV-2 spike glycoprotein on the surface of coronavirus-sized particles was denatured in 30 s by subtilisin enzyme-functionalized membranes with 0.02-0.2% water content on the membrane surface.