Development of a reverse genetics system enabling the rescue of recombinant avian influenza virus A/Turkey/England/50-92/91 (H5N1)
Development of a reverse genetics system enabling the rescue of recombinant avian influenza virus A/Turkey/England/50-92/91 (H5N1)
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DOI：
10.1637/7645-050906r1.1
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发表时间：
2007-01-01
期刊：
影响因子：
1.4
通讯作者：
Barclay, Wendy
中科院分区：
文献类型：
--
作者：
Howard, Wendy;Hayman, Anna;Barclay, Wendy
我们之前描述了利用已建立的反向遗传学系统从克隆的cDNA中产生重组人甲型流感病毒。在这里，我们组装了一组质粒，使H5N1禽流感病毒A/土耳其/英格兰/50-92/91完全从cDNA中恢复。这个系统使我们能够在cDNA中引入突变或截断，以创造在所选基因中特定改变的突变病毒。然后，这些突变病毒可以用于未来对鸡的致病机理研究，以及了解禽流感病毒在人类身上的宿主范围限制的研究。
We previously described the use of an established reverse genetics system for the generation of recombinant human influenza A viruses from cloned cDNAs. Here, we have assembled a set of plasmids to allow recovery of the avian H5N1 influenza virus A/Turkey/England/50-92/91 entirely from cDNA. This system enables us to introduce mutations or truncations into the cDNAs to create mutant viruses altered specifically in a chosen gene. These mutant viruses can then be used in future pathogenesis studies in chickens and in studies to understand the host range restrictions of avian influenza viruses in humans.