Disruption of the gene (spoOA) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A (Retracted Article)
Disruption of the gene (spoOA) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A (Retracted Article)
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DOI：
10.1016/j.femsle.2004.02.014
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发表时间：
2004-04-15
影响因子：
2.1
通讯作者：
Sarker, MR
中科院分区：
文献类型：
--
作者：
Huang, IH;Waters, M;Sarker, MR
本研究在产肠毒素A型产气荚膜梭菌中鉴定了一个功能性spo0A ORF。为了评估spo0A的功能，构建了spo0A敲除的等基因突变体。spo0A突变体不能形成内生孢子和产生肠毒素，然而，这些缺陷可以通过用携带野生型spo0A基因的重组质粒补充突变体来恢复。这些结果提供的证据表明，spo0A的表达是必不可少的产气荚膜梭菌的孢子形成和肠毒素的生产。(C)2004年，欧洲微生物学会联合会。Elsevier B.V.出版，保留所有权利。
This study identified a functional spo0A ORF in enterotoxigenic Clostridium perfringens type A. To evaluate the function of spo0A, an isogenic spo0A knock-out mutant was constructed. The spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wildtype spo0A gene. These results provide evidence that spo0A expression is essential for sporulation and enterotoxin production in C perfringens. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.