Emergence of NDM-1 Metallo-β-Lactamase in Pseudomonas aeruginosa Clinical Isolates from Serbia
Emergence of NDM-1 Metallo-β-Lactamase in Pseudomonas aeruginosa Clinical Isolates from Serbia
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DOI：
10.1128/aac.00226-11
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发表时间：
2011-08-01
影响因子：
4.9
通讯作者：
Kojic, Milan
中科院分区：
文献类型：
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作者：
Jovcic, Branko;Lepsanovic, Zorica;Kojic, Milan
这项工作首次报道了新德里金属β-内酰胺酶1(NDM-1)在铜绿假单胞菌中的存在。此外，这是关于NDM-1在巴尔干地区存在的第一份报告。对碳青霉烯类不敏感铜绿假单胞菌临床分离株MMA83和MMA533的Cosmid基因文库进行金属β-内酰胺酶检测。相应地，MMA83和MMA533均携带bla(NDM-1)基因。脉冲场凝胶电泳(PFGE)分析表明，菌株MMA83和MMA533属于不同的克隆群。来自不同患者的另外5株与MMA83或MMA533克隆性相关的分离株被发现NDM-1阳性。
This work reports, for the first time, the presence of New Delhi metallo-beta-lactamase 1 (NDM-1) in Pseudomonas aeruginosa. Moreover, this is the first report of the NDM-1 presence in the Balkan region. Cosmid gene libraries of carbapenem-nonsusceptible Pseudomonas aeruginosa clinical isolates MMA83 and MMA533 were screened for the presence of metallo-beta-lactamases. Accordingly, both MMA83 and MMA533 carried the bla(NDM-1) gene. Pulsed-field gel electrophoresis (PFGE) analysis indicated that strains MMA83 and MMA533 belonged to different clonal groups. Five additional isolates from different patients clonally related to either MMA83 or MMA533 were found to be NDM-1 positive.