Correlation between nec1 Gene Copy Number Detected in Soils by Quantitative Competitive Quenching Probe PCR and Incidence of Potato Common Scab Disease
Correlation between nec1 Gene Copy Number Detected in Soils by Quantitative Competitive Quenching Probe PCR and Incidence of Potato Common Scab Disease
复制标题
定量竞争淬灭探针PCR检测土壤中nec1基因拷贝数与马铃薯赤霉病发病率的相关性
DOI：
10.1264/jsme2.21.185
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发表时间：
2006
影响因子：
2.2
通讯作者：
Hideo Tanaka
中科院分区：
文献类型：
--
作者：
O. Koyama;A. Manome;S. Kurata;T. Yokomaku;Hideo Tanaka
本研究对长崎市54块马铃薯田2003年和2004年春播期间的土壤进行了调查，分析了收获时nec1基因的可检测拷贝数与各块马铃薯田结痂病发生的关系。利用nec1基因特异性引物和荧光猝灭探针，采用定量竞争猝灭探针PCR （QCQP-PCR）方法对土壤样品中的nec1基因进行定量分析。2003年21块地块茎中nec1基因拷贝数与发病呈显著正相关（r2=0.8195）。在25个发病率为10%的田中，有23个田中每克土壤检测到103个以上的nec1拷贝。在发病率<10%的9个领域中，8个领域的基因拷贝数少于103个。在未发现病害的20个大田中未检测到nec1基因。这些结果表明，在每g干土壤检测到103个以上nec1拷贝的田地中，结痂病发生的频率更高。
We surveyed the soils from 54 potato fields in Nagasaki during the 2003 and 2004 spring croppings, and examined the relationship between the detectable copy number of the nec1 gene at harvest and the incidence of scab disease in each field. The nec1 gene in soil samples was quantified by a quantitative competitive quenching probe PCR (QCQP-PCR) method, using primers specific to the nec1 gene and fluorescence quenching-based probes. A significant positive correlation (r2=0.8195) was observed between the detectable copy number of the nec1 gene and the incidence of disease in tubers from 21 fields in 2003. In 23 of 25 fields where the incidence of disease was >10%, more than 103 copies of nec1 per g-soil were detected. Of the nine fields where the incidence of disease was <10%, eight had fewer than 103 gene copies. The nec1 gene was not detected in 20 fields where no disease was found. These results suggest that scab disease occurs more frequently in fields where more than 103 copies of nec1 per g-dry soil are detected.