Alteration of the retinotectal map in Xenopus by antibodies to neural cell adhesion molecules.
Alteration of the retinotectal map in Xenopus by antibodies to neural cell adhesion molecules.
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神经细胞粘附分子抗体改变爪蟾视网膜顶盖图。
DOI：
10.1073/pnas.81.13.4222
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发表时间：
1984
影响因子：
11.1
通讯作者：
Edelman,GM
中科院分区：
文献类型：
--
作者：
Fraser,SE;Murray,BA;Chuong,CM;Edelman,GM
神经细胞粘附分子（N-CAM）介导神经元-神经元粘附，在发育和成熟脊椎动物的神经系统中普遍存在，并且在发育过程中其数量和分布发生重大变化。此前已发现，特异性抗 N-CAM 抗体的单价片段对同质性（N-CAM 至 N-CAM）结合的干扰可在体外改变神经组织模式。为了证明体内也可能发生显着的改变，将非洲爪蟾 N-CAM 抗体嵌入琼脂糖微圆柱中，并植入正在经历视网膜顶盖投影再生的幼年非洲爪蟾的顶盖； 1周后，确定植入对视神经投射模式的影响。 N-CAM 的多克隆和单克隆抗体都会扭曲视网膜顶盖投影模式，并大大降低投影的精度；三周后这些变化恢复到接近正常。当正常发育的幼蛙接受顶盖植入物时，也获得了类似但较小的效果。在对照动物中，植入来自免疫前血清的免疫球蛋白和不针对 N-CAM 的单克隆抗体对模式影响很小或没有影响。结果表明，N-CAM 介导的神经元粘附对于建立和维持神经模式的精度和拓扑结构非常重要。
The neural cell adhesion molecule (N-CAM) mediates neuron-neuron adhesion, is ubiquitous in the nervous system of developing and mature vertebrates, and undergoes major alterations in both amount and distribution during development. Perturbation of homophilic (N-CAM to N-CAM) binding by univalent fragments of specific anti-N-CAM antibodies has previously been found to alter neural tissue patterns in vitro. To show that significant alterations can also occur in vivo, antibodies to Xenopus N-CAM were embedded in agarose microcylinders and implanted in the tecta of juvenile Xenopus laevis frogs that were undergoing regeneration of their retinotectal projections; 1 week later, the effects of implantation on the projection pattern from the optic nerve were determined. Both polyclonal and monoclonal antibodies to N-CAM distorted the retinotectal projection pattern and greatly decreased the precision of the projection; these alterations recovered to near normal after an additional 3 weeks. Similar but smaller effects were obtained when normally developing froglets received tectal implants. In control animals, implants of immunoglobulins from preimmune serum and monoclonal antibodies not directed against N-CAM had little or no effect on the pattern. The results suggest that neuronal adhesion mediated by N-CAM is important in establishing and maintaining the precision and topography of neural patterns.