Platelet-derived growth factor-modulated proteins: constitutive synthesis by a transformed cell line.
Platelet-derived growth factor-modulated proteins: constitutive synthesis by a transformed cell line.
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血小板衍生的生长因子调节蛋白：转化细胞系的组成型合成。
DOI：
10.1073/pnas.78.7.4358
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发表时间：
1981
影响因子：
11.1
通讯作者：
Scher,CD
中科院分区：
文献类型：
--
作者：
Pledger,WJ;Hart,CA;Locatell,KL;Scher,CD
血小板衍生生长因子（PDGF）通过使密度停滞的BALB/c-3 T3小鼠细胞对血浆中所含因子产生应答而启动其复制。用PDGF处理静止细胞迅速刺激几种细胞质蛋白（分子量29，000至70，000）的优先合成。这些蛋白质中有四个在PDGF添加后1.5小时内被发现，一个在40分钟内被发现。RNA合成抑制剂阻止了这些蛋白质的合成。pI的合成和DNA合成的刺激都显示出对PDGF浓度的类似剂量反应。也可诱导感受态的PGFs刺激pI和pII的合成。血浆、表皮生长因子或胰岛素不会诱导感受态，也不会刺激这些蛋白质的选择性合成。BALB/c-3 T3细胞的转化变体保留了对血浆因子的生长要求，但失去了对PDGF的要求，组成性地合成这些PDGF调节的蛋白质。
Platelet-derived growth factor (PDGF) initiates replication of density-arrested BALB/c-3T3 mouse cells by rendering them "competent" to respond to factors contained in plasma. Treatment of quiescent cells with PDGF rapidly stimulates the preferential synthesis of several cytoplasmic proteins (molecular weights 29,000 to 70,000). Four of these proteins were noted within 1.5 hr of PDGF addition and one (pI) within 40 min. Inhibitors of RNA synthesis prevented the synthesis of these proteins. Both the synthesis of pI and the stimulation of DNA synthesis displayed a similar dose response to PDGF concentration. Pituitary fibroblast growth factor, which also induces competence, stimulated pI and pII synthesis. Plasma, epidermal growth factor, or insulin, which do not induce competence, did not stimulate selective synthesis of these proteins. A transformed variant of BALB/c-3T3 cells, which has retained the growth requirement for plasma factors but lost the requirement for PDGF, synthesizes these PDGF-modulated proteins constitutively.