Real-Time Molecular Imaging throughout the Entire Cell Cycle by Targeted Plasmonic-Enhanced Rayleigh/Raman Spectroscopy
Real-Time Molecular Imaging throughout the Entire Cell Cycle by Targeted Plasmonic-Enhanced Rayleigh/Raman Spectroscopy
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DOI：
10.1021/nl3027586
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发表时间：
2012-10-01
期刊：
影响因子：
10.8
通讯作者：
El-Sayed, Mostafa A.
中科院分区：
文献类型：
--
作者：
Kang, Bin;Austin, Lauren A.;El-Sayed, Mostafa A.
由于它们对散射光的强烈增强，等离子体纳米颗粒已被用于各种生物和医学应用。在这里，我们描述了一种新的技术，有针对性的等离子体增强单细胞瑞利/拉曼光谱，监测分子变化的任何细胞成分，如细胞核，在其完整的细胞周期的不同阶段，同时记录其瑞利图像和拉曼振动光谱实时。对观察到的拉曼DNA和蛋白质峰的分析允许识别细胞周期的不同阶段。这项技术可用于疾病诊断，并可能提高我们对细胞功能的分子机制的理解，如分裂，死亡，信号传导和药物作用。
Due to their strong enhancement of scattered light, plasmonic nanoparticles have been utilized for various biological and medical applications. Here, we describe a new technique, Targeted Plasmonic-Enhanced Single-Cell Rayleigh/Raman Spectroscopy, to monitor the molecular changes of any cell-component, such as the nucleus, during the different phases of its full cell cycle by simultaneously recording its Rayleigh images and Raman vibration spectra in real-time. The analysis of the observed Raman DNA and protein peaks allowed the different phases of the cell cycle to be identified. This technique could be used for disease diagnostics and potentially improve our understanding of the molecular mechanisms of cellular functions such as division, death, signaling, and drug action.