Adaptation of Cucumber mosaic virus soybean strains (SSVs) to cultivated and wild soybeans
Adaptation of Cucumber mosaic virus soybean strains (SSVs) to cultivated and wild soybeans
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DOI：
10.1007/s00122-003-1222-3
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发表时间：
2003-06-01
影响因子：
5.4
通讯作者：
Uyeda, I
中科院分区：
文献类型：
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作者：
Hong, JS;Masuta, C;Uyeda, I
为了解黄瓜花叶病毒（Cucumber mosaic virus，SSV）与大豆的共同进化，将大豆矮化病毒（soybean stunt virus，SSV）分离株接种到来自亚洲4个国家的23个野生大豆上，研究其侵染性。接种SSV后，大部分野生大豆都发生了系统感染。然而，在印度尼西亚分离的一种SSV株（SSV-In）并没有导致分布在日本南部的许多野生大豆的系统感染。SSV-In的这种特殊感染性可能是由于其对印度尼西亚当地大豆种群的特异性适应，而印度尼西亚当地大豆种群很少受到来自野生大豆的基因流的影响。本研究测定了黄瓜花叶病毒（Cucumber mosaic virus，CMV）3a和CP基因的核苷酸序列，并对7个已知的黄瓜花叶病毒分离物进行了分类。系统发育分析表明，7个SSVs形成了一个独特的聚类分离的其他CMV株，尽管他们的不同地理来源，SSV-In是最分歧的7个分离物。同义和非同义替换率的比较表明，SSV组的发展速度比IA亚组。研究结果的影响进行了讨论，在所谓的红皇后假说。
Cucumber mosaic virus soybean strains formerly called soybean stunt virus (SSV) were inoculated onto 23 wild soybeans collected from four Asian countries to investigate their infectivity in order to improve understanding of the co-evolution of SSVs and soybean. SSV inoculation resulted in systemic infection in most of the wild soybeans used. However, an SSV strain (SSV-In), which was isolated in Indonesia, did not result in systemic infection of many of the wild soybeans distributed in southern Japan. This exceptional infectivity of SSV-In may be due to its specific adaptation to the local soybean population(s) of Indonesia, which has rarely been affected by gene flows from wild soybean. In the present study, the nucleotide sequences of the 3a and CP genes of SSV were determined, and the data were used to classify seven SSV isolates among known Cucumber mosaic virus (CMV) strains. The phylogenetic analysis showed that the seven SSVs formed a distinct cluster separated from the other CMV strains despite their different geographical origins; SSV-In was the most divergent of the seven isolates. Comparison of the rates of synonymous and nonsynonymous substitutions revealed that the SSV group had evolved faster than subgroup IA. The implications of the findings are discussed in relation to the so-called Red Queen hypothesis.