Two types of local interneurons are distinguished by morphology, intrinsic membrane properties, and functional connectivity in the moth antennal lobe.
Two types of local interneurons are distinguished by morphology, intrinsic membrane properties, and functional connectivity in the moth antennal lobe.
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两种类型的局部中间神经元通过形态、内在膜特性和蛾触角叶的功能连接来区分。
DOI：
10.1152/jn.00050.2015
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发表时间：
2015
影响因子：
2.5
通讯作者：
and Ryohei Kanzaki
中科院分区：
文献类型：
--
作者：
Masashi Tabuchi1;Li Dong;Shigeki Inoue;Shigehiro Namiki;Takeshi Sakurai;Kei Nakatani;and Ryohei Kanzaki
家蚕触角叶（AL）的神经元的形态和气味诱发的放电活动的特点。然而，其内在的电特性，包括电压门控离子电流和突触连接仍然不清楚。为了解决这个问题，全细胞电流和电压钳记录从二级投射神经元（PN）和两种形态类型的本地中间神经元（LN）在蚕蛾AL。这两种形态类型的LN表现出不同的生理特性。一种形态学类型的LN表现为电压门控钠通道基因表达的尖峰反应，而另一种类型的LN是无电压门控钠通道基因表达的非尖峰反应。电压钳实验还显示，两种类型的LN和PN都具有两种类型的电压门控性钾通道和钙通道。在尖峰LN和PN的双全细胞记录中，PN的激活引起配对尖峰LN中的去极化反应，而尖峰LN的激活在配对PN中不引起实质性反应。然而，同时记录的nonspiking LN和PN表明，激活nonspiking LN引起的超极化反应的PN。我们还观察到双向突触传递通过化学和电耦合的尖峰LNs对。因此，我们的研究结果表明，有两种不同类型的LNs在家蚕AL，和它们的功能连接到PNs有很大的不同。我们提出了不同的功能作用，这两种不同类型的LN在塑造气味诱发的放电活动在PN。
Neurons in the silkmoth antennal lobe (AL) are well characterized in terms of their morphology and odor-evoked firing activity. However, their intrinsic electrical properties including voltage-gated ionic currents and synaptic connectivity remain unclear. To address this, whole cell current- and voltage-clamp recordings were made from second-order projection neurons (PNs) and two morphological types of local interneurons (LNs) in the silkmoth AL. The two morphological types of LNs exhibited distinct physiological properties. One morphological type of LN showed a spiking response with a voltage-gated sodium channel gene expression, whereas the other type of LN was nonspiking without a voltage-gated sodium channel gene expression. Voltage-clamp experiments also revealed that both of two types of LNs as well as PNs possessed two types of voltage-gated potassium channels and calcium channels. In dual whole cell recordings of spiking LNs and PNs, activation of the PN elicited depolarization responses in the paired spiking LN, whereas activation of the spiking LN induced no substantial responses in the paired PN. However, simultaneous recording of a nonspiking LN and a PN showed that activation of the nonspiking LN induced hyperpolarization responses in the PN. We also observed bidirectional synaptic transmission via both chemical and electrical coupling in the pairs of spiking LNs. Thus our results indicate that there were two distinct types of LNs in the silkmoth AL, and their functional connectivity to PNs was substantially different. We propose distinct functional roles for these two different types of LNs in shaping odor-evoked firing activity in PNs.