Intracellular Nanoparticle Coating Stability Determines Nanoparticle Diagnostics Efficacy and Cell Functionality
Intracellular Nanoparticle Coating Stability Determines Nanoparticle Diagnostics Efficacy and Cell Functionality
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DOI：
10.1002/smll.201000763
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发表时间：
2010-10-04
期刊：
影响因子：
13.3
通讯作者：
De Cuyper, Marcel
中科院分区：
文献类型：
--
作者：
Soenen, Stefaan J. H.;Himmelreich, Uwe;De Cuyper, Marcel
氧化铁纳米颗粒 (NP) 经常在生物医学研究中用作磁共振 (MR) 造影剂，其中需要高细胞内水平才能清楚地描绘信号变化。迄今为止，这些颗粒的毒性和适用性尚未完全阐明。在这里，我们表明不同氧化铁颗粒的内体定位导致它们降解并降低 MR 对比度，其速率主要由涂层的稳定性决定。三价铁的释放会产生活性物质，极大地影响细胞功能。脂质包被的纳米粒子表现出最高的稳定性，并且还表现出细胞内聚集，这显着增强了它们的MR特性和细胞内持久性。这些发现非常重要，因为根据涂层的性质，颗粒可能会迅速降解，从而完全消除其 MR 对比，使其达到与对照相比不可检测的水平，并极大地阻碍细胞功能，从而阻碍它们在功能性体内研究中的应用。
Iron oxide nanoparticles (NPs) are frequently employed in biomedical research as magnetic resonance (MR) contrast agents where high intracellular levels are required to clearly depict signal alterations. To date, the toxicity and applicability of these particles have not been completely unraveled. Here, we show that endosomal localization of different iron oxide particles results in their degradation and in reduced MR contrast, the rate of which is governed mainly by the stability of the coating. The release of ferric iron generates reactive species, which greatly affect cell functionality. Lipid-coated NPs display the highest stability and furthermore exhibit intracellular clustering, which significantly enhances their MR properties and intracellular persistence. These findings are of considerable importance because, depending on the nature of the coating, particles can be rapidly degraded, thus completely annihilating their MR contrast to levels not detectable when compared to controls and greatly impeding cell functionality, thereby hindering their application in functional in vivo studies.