Effect of Oral Zinc Supplementation on the Thiol Oxido-Reductive Index and Thiol-Related Enzymes in Seminal Plasma and Spermatozoa of Iraqi Asthenospermic Patients
Effect of Oral Zinc Supplementation on the Thiol Oxido-Reductive Index and Thiol-Related Enzymes in Seminal Plasma and Spermatozoa of Iraqi Asthenospermic Patients
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DOI：
10.1007/s12011-017-1215-8
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发表时间：
2018-08-01
影响因子：
3.9
通讯作者：
Hadwan, Mahmoud Hussein
中科院分区：
文献类型：
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作者：
Alsalman, Abdul Razzaq S.;Almashhedy, Lamia A.;Hadwan, Mahmoud Hussein
硫醇基在精子代谢和抗氧化防御状态中起着重要作用。锌是人体内含量第二丰富的元素，仅次于铁。本研究旨在研究补锌对弱精子症患者精液特征以及硫醇和硫醇相关酶的影响。精液样本取自 60 名有生育能力的男性和 60 名弱精子症男性，这些男性曾在巴比尔医院（伊拉克希拉市）不孕不育诊所就诊。生育力低下组接受锌治疗；每位参与者每天服用两粒 220 毫克硫酸锌胶囊，持续 3 个月。获取精液样本（补充锌之前和之后）。测定患者和健康组精子和精浆中还原硫醇、氧化硫醇、硫醇氧化还原指数和硫醇相关酶活性的水平。与生育组相比，不育患者的氧化硫醇水平显着较高。相反，与生育组相比，不育患者的还原硫醇水平、巯基氧化酶活性和谷胱甘肽过氧化物酶活性显着降低。不孕症患者接受锌治疗后，氧化硫醇水平、还原硫醇水平以及硫醇相关酶活性均恢复至正常值。然而，在锌处理组中，精子中还原硫醇和氧化硫醇的水平没有显着变化。 RSH/RSSR 和硫醇相关酶的定量值可以为临床和流行病学研究中定性表达氧化剂/抗氧化剂平衡提供有用的手段。 ClinicalTrials.gov 标识符：NCT02985905。
A thiol group plays an essential role in sperm metabolism and the antioxidative defense state. Zinc is the second most abundant element in the human body, following iron. The present study was conducted to study the effect of zinc supplementation on the characteristics of semen along with thiol and thiol-related enzymes in semen of asthenospermic patients. Semen samples were obtained from 60 fertile and 60 asthenospermic men, from couples who had consulted the infertility clinic of Babil Hospital (Hillah city, Iraq). The subfertile group was treated with zinc; every participant took two 220 mg capsules of zinc sulfate per day for 3 months. Semen samples were obtained (before and after zinc supplementation). The levels of reduced thiol, oxidized thiol, thiol oxido-reductive index, and thiol-related enzymes activities were determined in spermatozoa and seminal plasma of patients and healthy groups. Oxidized thiol levels were significantly higher in the infertile patients compared to that in the fertile group. Conversely, reduced thiol level, sulfhydryl oxidase activity, and glutathione peroxidase activity significantly decreased in the infertile patients compared to that in the fertile group. Oxidized thiol levels, reduced thiol levels, and thiol-related enzymes activities of the infertile patients were restored to normal values after treatment with zinc. However, reduced and oxidized thiol levels in spermatozoa did not change significantly in the group treated with zinc. The quantitative values for RSH/RSSR and thiol-related enzymes may provide useful means to qualitatively express the oxidant/antioxidant balance in clinical and epidemiologic studies. ClinicalTrials.gov Identifier: NCT02985905.