Spontaneous burrowing behaviour in the rat is reduced by peripheral nerve injury or inflammation associated pain
Spontaneous burrowing behaviour in the rat is reduced by peripheral nerve injury or inflammation associated pain
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DOI：
10.1016/j.ejpain.2011.07.012
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发表时间：
2012-04-01
影响因子：
3.6
通讯作者：
Rice, A. S. C.
中科院分区：
文献类型：
--
作者：
Andrews, N.;Legg, E.;Rice, A. S. C.
疼痛影响日常生活的许多方面，有效的止痛药应恢复正常的自发日常行为。本文描述的实验表明，大鼠先天的自发的打洞行为可通过测量在给予大鼠1小时后留在中空管中的砾石量来简单和客观地评估，周围神经损伤降低了这种行为（胫神经横断（TNT），L5脊神经横断（SNT）和部分坐骨神经结扎（PSNL））以及足底内注射完全弗氏佐剂（CFA）诱导的炎症后。加巴喷丁（100 mg/kg皮下注射）（但30 mg/kg皮下注射）显着降低了幼稚大鼠的穴居活动。所有周围神经损伤和CFA减少洞穴相比，假手术和大鼠幼稚的手术。周围神经损伤大鼠的机械超敏反应水平与穴居缺陷无关，表明在这些范例中测量了整体疼痛体验的不同参数。加巴喷丁在30 mg/kg sc，但不是100 mg/kg sc，逆转由TNT和布洛芬（30 mg/kg sc）诱导的洞穴的缺陷逆转CFA对洞穴的影响。这些实验表明，穴居的测量是一个简单的，客观的测定先天啮齿动物的行为受疼痛的影响，是行为学相关的大鼠，不完全依赖于唤起反射，并可以分离选择性镇痛剂量的加巴喷丁从一个诱导运动障碍在同一动物。
Pain influences many aspects of daily living and effective analgesics should reinstate normal spontaneous daily behaviours. Experiments are described herein which show that the innate, spontaneous behaviour of burrowing by rats, which can be simply and objectively assessed by measuring the amount of gravel left in a hollow tube 1 h after presentation to the rat, is reduced by peripheral nerve injury (tibial nerve transection (TNT), L5 spinal nerve transection (SNT) and partial sciatic nerve ligation (PSNL)) and also following inflammation induced by intra-plantar injection of Complete Freund's Adjuvant (CFA). Gabapentin (100 mg/kg sc) but not at 30 mg/kg sc significantly reduced burrowing activity in naive rats. All peripheral nerve injuries and CFA reduced burrowing compared with shams and rats naive to surgery. The level of mechanical hypersensitivity in rats with peripheral nerve injury did not correlate with the deficit in burrowing indicating that different parameters of the holistic pain experience are measured in these paradigms. Gabapentin at 30 mg/kg sc, but not 100 mg/kg sc, reversed the deficit in burrowing induced by TNT and ibuprofen (30 mg/kg sc) reversed the effect of CFA on burrowing. These experiments show that measurement of burrowing is a simple, objective assay of innate rodent behaviour affected by pain that is ethologically relevant to the rat, does not rely wholly on evoking a reflex and can dissociate a selective analgesic dose of gabapentin from one inducing motor impairment in the same animal.