Prodigiosin Induces Autolysins in Actively Grown Bacillus subtilis Cells.
Prodigiosin Induces Autolysins in Actively Grown Bacillus subtilis Cells.
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DOI：
10.3389/fmicb.2016.00027
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发表时间：
2016
影响因子：
5.2
通讯作者：
Stopar D
中科院分区：
文献类型：
--
作者：
Danevčič T;Borić Vezjak M;Tabor M;Zorec M;Stopar D
海洋细菌橡胶弧菌DSM 14379产生的Prodigiosin对多种革兰氏阳性和革兰氏阴性细菌具有有效的抗菌活性。然而，其抗菌作用机制尚不清楚。本文研究了芥子油苷对枯草芽孢杆菌生长、细胞膜渗漏和诱导自溶素的影响。用芥子红素处理枯草芽孢杆菌后，其光密度迅速下降，β-半乳糖苷酶活性测定的细胞膜渗漏量增加。在自溶酶基因lytABCD受损的突变株中，与野生型菌株相比，其裂解活性下降了80%，而在lytABCDEF突变株中，prodigiosin没有溶菌作用，只有抑菌作用。改良的彗星试验证实了对枯草芽孢杆菌细胞的快速裂解活性。结果表明，枯草芽孢杆菌对芥子菌素的诱导是生长阶段依赖性的。
Prodigiosin produced by marine bacterium Vibrio ruber DSM 14379 exhibits a potent antimicrobial activity against a broad range of Gram positive and Gram negative bacteria. The mechanism of prodigiosin antimicrobial action, however, is not known. In this work, the effect of prodigiosin on Bacillus subtilis growth, cell membrane leakage, and induction of autolysins was studied. Treating B. subtilis with prodigiosin resulted in rapid decline of optical density and increased cell membrane leakage measured by β-galactosidase activity. Cell lysis was initiated immediately after treatment with prodigiosin in the middle exponential phase and was completed within 2 h. Lytic activity of prodigiosin in mutant strains with impaired autolysin genes lytABCD decreased for 80% compared to the wild type strain, while in lytABCDEF mutant strain prodigiosin had no bacteriolytic but only bacteriostatic effect. Fast prodigiosin lytic activity on individual B. subtilis cells was confirmed by a modified comet assay. The results indicate that prodigiosin autolysin induction in B. subtilis is growth phase dependent.