Cycle training modulates satellite cell and transcriptional responses to a bout of resistance exercise.
Cycle training modulates satellite cell and transcriptional responses to a bout of resistance exercise.
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DOI：
10.14814/phy2.12973
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发表时间：
2016-09
影响因子：
2.5
通讯作者：
Peterson CA
中科院分区：
文献类型：
--
作者：
Murach KA;Walton RG;Fry CS;Michaelis SL;Groshong JS;Finlin BS;Kern PA;Peterson CA
这项研究评估了中等强度的自行车运动是否会影响中年女性对急性最大向心/离心阻力运动的卫星细胞和分子反应。7名健康中年女性（56 ± 5岁，BMI 26 ± 1，VO 2 max 27 ± 4）在12周周期训练前后进行了基线和72小时抗阻运动后股外侧肌活检。通过免疫组织化学测定肌球蛋白重链（MyHC）I和II相关卫星细胞密度和横截面积。还通过NanoString测量了代表肌肉重塑环境的93个基因的表达。随着循环训练，总体纤维尺寸增加~20%（P = 0.052）。急性抗阻运动使心肌肌球蛋白I卫星细胞密度增加29%，耐力训练使心肌肌球蛋白I卫星细胞密度增加50%（P < 0.05）。耐力训练后，急性抗阻运动使肌球蛋白I卫星细胞密度下降13%（急性抗阻×训练交互作用，P < 0.05）。具有相互作用效应的基因与卫星细胞行为一起跟踪，在未训练状态下增加，在耐力训练状态下减少，以响应阻力运动。类似的卫星细胞和基因表达反应模式表明协调调节肌肉环境，以促进适应。中等强度的耐力周期训练调节对急性阻力运动的反应，可能会使肌肉适应更强烈的同心/离心活动。这些结果表明，自行车训练是一种有效的耐力运动方式，可促进中年女性的生长，这些女性随着年龄的增长容易出现肌肉质量损失。
This investigation evaluated whether moderate‐intensity cycle ergometer training affects satellite cell and molecular responses to acute maximal concentric/eccentric resistance exercise in middle‐aged women. Baseline and 72 h postresistance exercise vastus lateralis biopsies were obtained from seven healthy middle‐aged women (56 ± 5 years, BMI 26 ± 1, VO 2max 27 ± 4) before and after 12 weeks of cycle training. Myosin heavy chain (MyHC) I‐ and II‐associated satellite cell density and cross‐sectional area was determined via immunohistochemistry. Expression of 93 genes representative of the muscle‐remodeling environment was also measured via NanoString. Overall fiber size increased ~20% with cycle training (P = 0.052). MyHC I satellite cell density increased 29% in response to acute resistance exercise before endurance training and 50% with endurance training (P < 0.05). Following endurance training, MyHC I satellite cell density decreased by 13% in response to acute resistance exercise (acute resistance × training interaction, P < 0.05). Genes with an interaction effect tracked with satellite cell behavior, increasing in the untrained state and decreasing in the endurance trained state in response to resistance exercise. Similar satellite cell and gene expression response patterns indicate coordinated regulation of the muscle environment to promote adaptation. Moderate‐intensity endurance cycle training modulates the response to acute resistance exercise, potentially conditioning the muscle for more intense concentric/eccentric activity. These results suggest that cycle training is an effective endurance exercise modality for promoting growth in middle‐aged women, who are susceptible to muscle mass loss with progressing age.