Molecular analysis of Plasmodium vivax relapses using the MSP1 molecule as a genetic marker
Molecular analysis of Plasmodium vivax relapses using the MSP1 molecule as a genetic marker
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DOI：
10.1086/517389
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发表时间：
1998-02-01
影响因子：
6.4
通讯作者：
del Portillo, HA
中科院分区：
文献类型：
--
作者：
Kirchgatter, K;del Portillo, HA
间日疟原虫有肝细胞休眠阶段，催眠子，引起复发。本研究使用裂殖子表面蛋白1基因PvMSP1作为遗传标记，比较了从巴西10个个体中获得的初发和复发的成对分离株。原发攻击的4个样本含有两种主要PvMSP1等位基因形式的基因混合寄生虫。PCR结果显示，2例患者的复发寄生物中存在这2种形式，表明催眠子的激活不是克隆的。来自配对初发/复发样本的DNA序列表明，来自初次攻击的寄生虫与复发样本中的寄生虫相同，其中在两种感染中检测到相同的等位基因形式。对这些患者针对表达c端PvMSP1的重组蛋白的自然获得性体液免疫反应的研究表明，在复发期间，IgG1亚类的滴度、亲和力成熟和优势增加。
Plasmodium vivax has hepatocytic dormant stages, hypnozoites, that cause relapses. This work compared paired isolates from primary attacks and relapses obtained from 10 individuals in Brazil using the merozoite surface protein 1 gene, PvMSP1, as a genetic marker. Four samples from primary attacks contained genetically mixed parasites harboring the 2 major PvMSP1 allelic forms. PCR revealed the presence of these 2 forms in the relapse parasites of 2 patients, demonstrating that the activation of hypnozoites is not clonal. DNA sequences from paired primacy/relapse samples demonstrated that the parasites from the primary attack are identical to those in relapse samples in which the same allele forms were detected in both infections. Studies on the naturally acquired humoral immune responses of these patients against a recombinant protein expressing the C-terminus PvMSP1 demonstrated an increase in the titers, affinity maturation, and predominance of the IgG1 subclass during the relapse.