Expression and immunolocalization of water-channel aquaporins in the rat and mouse mammary gland
Expression and immunolocalization of water-channel aquaporins in the rat and mouse mammary gland
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DOI：
10.1007/s00418-005-0753-x
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发表时间：
2005-06-01
影响因子：
2.3
通讯作者：
Takata, K
中科院分区：
文献类型：
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作者：
Matsuzaki, T;Machida, N;Takata, K
我们通过逆转录酶聚合酶链反应（RT-PCR）、免疫印迹和免疫组织化学检查了乳腺中水通道水通道蛋白的表达和免疫定位。RT-PCR和免疫印迹法检测水通道蛋白1（AQP 1）和AQP 3在泌乳大鼠乳腺中的表达。AQP 3主要分布于肺泡上皮和导管系统，而AQP 1主要分布于毛细血管和微静脉。AQP 3表达于分泌上皮细胞、小叶内和小叶间导管上皮细胞的基底膜。乳头口附近的主导管由复层上皮组成，其基底层和中间层含有AQP 3。AQP 1定位于毛细血管和微静脉内皮细胞的顶膜和基底膜。AQP 3在处女女性中未检测到。AQP 3存在于妊娠大鼠一些正在分化的乳腺上皮细胞中。AQP 1存在于毛细血管和微静脉在分化的乳腺的怀孕大鼠和处女女性的乳腺脂肪垫。我们发现AQP 1和AQP 3在小鼠中的分布相似。AQP 1和AQP 3似乎在乳汁的合成和/或分泌中起作用。
We examined the expression and immunolocalization of water-channel aquaporins in the mammary gland by reverse transcriptase polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemistry. RT-PCR and immunoblotting revealed the expression of aquaporin-1 (AQP1) and AQP3 in the lactating rat mammary gland. AQP3 was detected in the alveolar epithelium and duct system whereas AQP1 was found in the capillaries and venules. AQP3 was present in the basolateral membrane of secretory epithelial cells and intralobular and interlobular duct epithelial cells. The main duct near the orifice in the nipple, which is comprised of a stratified epithelium, bore AQP3 in its basal and intermediate layers. AQP1 was located in both the apical and basolateral membranes of capillary and venule endothelia. AQP3 was not detected in virgin females. AQP3 was found in some differentiating mammary epithelial cells in the pregnant rat. AQP1 was present in capillaries and venules in the differentiating mammary gland of the pregnant rat and in the mammary fat pad of virgin females. We found a similar distribution of AQP1 and AQP3 in the mouse. AQP1 and AQP3 seem to play roles in the synthesis and/or secretion of milk.