pH responsive glycopolymer nanoparticles for targeted delivery of anti-cancer drugs
pH responsive glycopolymer nanoparticles for targeted delivery of anti-cancer drugs
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DOI：
10.1039/c7me00086c
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发表时间：
2018-02
期刊：
影响因子：
37.8
通讯作者：
G. Yilmaz;E. Guler;Caner Geyik;B. Demir;M. Ozkan;Dilek Odaci Demirkol;S. Ozcelik;S. Timur;C. Becer
中科院分区：
文献类型：
--
作者：
G. Yilmaz;E. Guler;Caner Geyik;B. Demir;M. Ozkan;Dilek Odaci Demirkol;S. Ozcelik;S. Timur;C. Becer
在过去的十年中，人们对纳米技术和碳水化合物的整合产生了极大的兴趣。糖纳米技术的进步已经允许为不同类型的医学应用，特别是药物递送和释放系统创建不同的生物活性糖纳米结构。因此，使用具有高负载能力、低总体毒性和受体介导的内吞作用特异性的更有效的生物相容性纳米载体仍然是增强治疗效果的焦点。将糖衍生物结合到金纳米颗粒上呈现出独特的性质，包括广泛的组装模型和与尺寸相关的电子、磁性和光学性质。在这里，pH响应性药物缀合的糖共聚物涂层的金纳米粒子制备的功能化的金纳米粒子与硫醇封端的糖共聚物，然后随后的阿霉素（DOX）的共轭。在四种不同的糖共聚物，他们的药物释放，物理化学特性（光谱，粒径和表面电荷）和体外生物应用与四种不同的细胞系进行了比较。因此，由于糖共聚物和DOX之间的腙键，通过糖包被的AuNP进行pH敏感的药物递送。比较活力测试还通过荧光细胞成像证明了甘醇共聚物-DOX缀合物的效率。所获得的结果揭示了AuNP均聚物DOX缀合物（P4 D）具有显著的潜力，特别是与宫颈癌细胞和肺癌细胞相比在人神经母细胞瘤细胞中。
Over the past decade, there has been a great deal of interest in the integration of nanotechnology and carbohydrates. The advances in glyconanotechnology have allowed the creation of different bioactive glyconanostructures for different types of medical applications, especially for drug delivery and release systems. Therefore, the use of more efficient biocompatible nanocarriers with high loading capacity, low overall toxicity and receptor-mediated endocytosis specificity is still in focus for the enhancement of the therapeutic effect. Conjugation of sugar derivatives onto gold nanoparticles presents unique properties that include a wide array of assembling models and size-related electronic, magnetic and optical properties. Here, pH-responsive drug-conjugated glycopolymer-coated gold nanoparticles were prepared by functionalization of gold nanoparticles with thiol-terminated glycopolymers and then subsequent conjugation of doxorubicin (DOX). Among the four different glycopolymers, their drug release, physicochemical characterization (spectroscopy, particle size and surface charge) and in vitro bioapplications with four different cell lines were compared. As a result, pH-sensitive drug delivery via sugar-coated AuNPs was performed thanks to hydrazone linkages between glycopolymers and DOX. Comparative viability tests also demonstrated the efficiency of glycopolymer–DOX conjugates by fluorescence cell imaging. The obtained results reveal that AuNP homoglycopolymer DOX conjugates (P4D) have significant potential, especially in human neuroblastoma cells in comparison to cervical cancer cells and lung cancer cells.