The change tendency of endoplasmic reticulum stress associated proteins in rats with spinal cord injury
The change tendency of endoplasmic reticulum stress associated proteins in rats with spinal cord injury
复制标题
脊髓损伤大鼠内质网应激相关蛋白的变化趋势
DOI：
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发表时间：
2019
影响因子：
2.2
通讯作者：
Yan Xinfeng
中科院分区：
文献类型：
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作者：
Zhu Yonglin;Zhang Luping;Fu Rongzhan;Gao Limin;Feng Guoying;Du Chao;Wang Zhaojie;Yan Xinfeng
通过葡萄糖调节蛋白78（GRP78）、C/EBP同源转录因子蛋白（CHOP）、X-box结合蛋白1 （XBP1）、Eif-2α和Bad的表达，探讨脊髓损伤大鼠内质网（ER）应激反应。用成年雌性小鼠建立脊髓损伤模型。脊髓损伤后，应用免疫组织化学和western blot检测小鼠内质网应激诱导凋亡蛋白在特定时间点的表达。免疫组化结果显示，在脊髓灰质中，细胞胞浆中特异性检测到Chop、Grp78、XBP1、Eif-2α和Bad。与脊髓损伤组比较，正常组和假手术组表达较少。脊髓损伤后内质网应激诱导的凋亡蛋白表达量显著升高，且绝对表达量高于正常组（P < 0.05）。Western-Blot结果显示，与脊髓损伤组相比，正常组和假手术组内质网应激诱导的凋亡蛋白表达较少。脊髓损伤后内质网应激诱导的凋亡蛋白表达量显著升高，且绝对表达量高于正常组（P < 0.05）。这些结果表明，一些内质网应激诱导的凋亡蛋白，如Chop、Grp78、XBP1、Eif-2α和Bad，在脊髓损伤后被激活，但确切的调控机制尚不清楚。在未来，了解内质网应激介导的脊髓损伤细胞凋亡的确切机制可能会导致新的治疗策略的发展。
To investigate endoplasmic reticulum (ER) stress reactions in spinal cord injury rats by evaluating the expression of the glucose-regulated protein 78(GRP78), C/EBP homologous transcription factor protein (CHOP), X-box binding protein 1 (XBP1), Eif-2α and Bad. SCI models were established using adult female mice. After SCI, the expression of endoplasmic reticulum stress-induced apoptosis proteins were examined in the mice at specific time points using immunohistochemistry and western blot. The results of immunohistochemistry showed that in spinal cord gray matter, Chop, Grp78, XBP1, Eif-2α and Bad were specifically detected in the cytoplasm of the cell. Compare with the SCI group, there was little expression in normal group and sham group. The expression of ER stress-induced apoptosis proteins were significantly increased after spinal cord injury, and the absolute expression was higher than normal group (P < 0.05). Western-Blot results showed that compare with the SCI group, there were little expression of ER stress-induced apoptosis proteins in normal group and sham group. The expression of ER stress-induced apoptosis proteins were significantly increased after spinal cord injury, and the absolute expression was higher than normal group (P < 0.05). These results suggest that some ER stress-induced apoptosis proteins, such as Chop, Grp78, XBP1, Eif-2α and Bad, were activated after spinal cord injury, but the precise regulatory mechanisms remain unclear. In the future, understanding of the precise mechanism of ER stress-mediated apoptosis in SCI may lead to the development of novel treatment strategies.