Development of a single-cassette system for spatiotemporal gene regulation in mice
Development of a single-cassette system for spatiotemporal gene regulation in mice
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DOI：
10.1016/j.bbrc.2005.10.054
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发表时间：
2005-12-16
影响因子：
3.1
通讯作者：
Miyazaki, J
中科院分区：
文献类型：
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作者：
Miyazaki, S;Miyazaki, T;Miyazaki, J
四环素调控的基因表达系统已被广泛用于小鼠中，以在靶器官中打开和关闭转基因，但只有有限的成功。我们开发了一种先进的系统，其中Tet-off调节单元被整合到ROSA 26基因座中，并在Cre介导的neo（r）基因切除后变得活跃。我们通过同源结构域转录因子pdx-1和增强型绿色荧光蛋白的可调控表达研究了该系统的实用性。由此产生的小鼠在neo（r）基因被移除的所有器官中显示出严格的四环素可调控的基因表达。当与器官特异性Cre重组酶转基因小鼠结合时，我们的系统允许我们以器官特异性和时间方式操纵基因表达。这种Tet-off系统应该作为分析基因在复杂生物系统中的作用的有效工具，例如胚胎发生、代谢、免疫系统等。All rights reserved.
The tetracycline-regulated gene expression system has been widely used in mice to turn a transgene on and off in a target organ, but with only limited success. We developed an advanced system in which a Tet-off regulation unit was integrated into the ROSA26 locus and became active after Cre-mediated excision of the neo(r) gene. We examined the utility of this system through regulable expression of the homeodomain transcription factor pdx-1 and enhanced green fluorescent protein. The resulting mice showed strict tetracycline-regulable gene expression in all the organs where the neo(r) gene had been removed. When combined with organ-specific Cre recombinase transgenic mice, our system allows us to manipulate the gene expression in an organ-specific and temporal manner. This Tet-off system should serve as an efficient tool to analyze the roles of genes in complex biological systems, such as embryogenesis, metabolism, immune system, etc. (c) 2005 Elsevier Inc. All rights reserved.