microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index
microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index
复制标题
DOI：
10.3389/fendo.2020.00206
复制
发表时间：
2020-04-28
影响因子：
5.2
通讯作者：
Atkin, Stephen L.
中科院分区：
文献类型：
--
作者：
Butler, Alexandra E.;Ramachandran, Vimal;Atkin, Stephen L.
背景：尽管有几位作者假设小分子非编码RNA（miR）的改变与多囊卵巢综合征（PCOS）的发病机制有关，但迄今为止已有相反的研究结果报道。体重指数（BMI）水平的差异可能是这些差异的原因;因此，本研究的目的是确定从年龄和BMI匹配的对照组和PCOS妇女收集的血清样本中miR是否不同。方法：在一项横断面研究中，使用定量聚合酶链反应对来自当地生物库的29名无排卵PCOS妇女和29名处于月经周期卵泡期的对照妇女的miR进行测定。结果：检测到176个miR，其中15个miR通过了PCOS和对照妇女之间差异的错误发现率（FDR;p< 0.05）。在PCOS或正常女性中，前9个miR（p< 0.02）（miR-486- 5 p、miR-24- 3 p、miR-19 b-3 p、miR-22- 3 p、miR-19 a-3 p、miR-339- 5 p、miR-185- 5 p、miR-101- 3 p、miR-let-7i-5 p）与BMI、雄激素水平、胰岛素抵抗或抗副中肾管激素（AMH）均无关联。对生殖系统异常的影响途径进行了评估，结果显示这些途径与生殖系统异常相关。结论：当考虑体重的混杂影响时，无排卵PCOS妇女和对照妇女在月经周期的卵泡期的miR水平存在差异。有趣的是，不同的miR与生殖异常的途径相关，但与AMH或代谢参数无关。
Background:Despite several authors who have hypothesized that alterations of small noncoding RNAs (miR) are implicated in the etiopathogenesis of polycystic ovarian syndrome (PCOS), contrasting findings have been reported so far. Discrepancies in body mass index (BMI) levels may account for these differences; therefore, the aim of the present study was to determine whether miR differed in serum samples collected from age- and BMI-matched control and PCOS women. Methods:In a cross-sectional study, miR were measured using quantitative polymerase chain reaction in 29 women with anovulatory PCOS women and 29 control women who were in the follicular phase of their menstrual cycle, from the local biobank. Results:One hundred seventy-six miR were detected, of which 15 miR passed the false discovery rate (FDR;p< 0.05) that differed between PCOS and control women. There was no association of the top 9 miR (p< 0.02) (miR-486-5p, miR-24-3p, miR-19b-3p, miR-22-3p, miR-19a-3p, miR-339-5p, miR-185-5p, miR-101-3p, miR-let-7i-5p) with BMI, androgen levels, insulin resistance, or antimullerian hormone (AMH) in either PCOS or normal women. Ingenuity pathway assessment showed the pathways were interrelated for abnormalities of the reproductive system. Conclusion:When the confounding influence of weight was accounted for, miR levels differed between anovulatory PCOS women and control women in the follicular phase of the menstrual cycle. Interestingly, the differing miR were associated with the pathways of reproductive abnormalities but did not associate with AMH or metabolic parameters.